CAJ | 학술논문

目的：研究不同浓度的酒精对斑马鱼的神经前体细胞、神经元和神经胶质细胞的影响。方法在斑马鱼卵受精后5 h，在斑马鱼培养液中加1%、2%或2.5%的酒精。采用原位杂交和实时定量PCR的方法检测不同细胞的mRNA水平的变化，以反映酒精对斑马鱼胚胎神经发育的影响。结果不同浓度酒精处理后，斑马鱼胚胎神经前体细胞和神经元显著减少，并且随着酒精浓度的增加其数量减少更加显著。早期的神经胶质细胞标志物的表达随着酒精浓度的增加在尾部减少，而在头部增加。成熟的神经胶质细胞标志物表达随着酒精浓度的增加而显著减少。结论酒精可以导致斑马鱼胚胎神经前体细胞、神经元和胶质细胞数量减少，并抑制神经元和神经胶质细胞的分化。
목적：연구불동농도적주정대반마어적신경전체세포、신경원화신경효질세포적영향。방법재반마어란수정후5 h，재반마어배양액중가1%、2%혹2.5%적주정。채용원위잡교화실시정량PCR적방법검측불동세포적mRNA수평적변화，이반영주정대반마어배태신경발육적영향。결과불동농도주정처리후，반마어배태신경전체세포화신경원현저감소，병차수착주정농도적증가기수량감소경가현저。조기적신경효질세포표지물적표체수착주정농도적증가재미부감소，이재두부증가。성숙적신경효질세포표지물표체수착주정농도적증가이현저감소。결론주정가이도치반마어배태신경전체세포、신경원화효질세포수량감소，병억제신경원화신경효질세포적분화。
Objective To investigate the influence of exposure to different concentrations of ethanol on neural progenitor cells and the differentiation of neurons and glial cells in zebrafish embryos. Methods Zebrafish embryos were exposed to 1%, 2%, and 2.5%(V/V) ethanol at 5 hpf by adding ethanol to the egg water. In situ hybridization and real-time PCR were used to detect the changes in the mRNA expression profiles of the markers of different cells to examine the effects of alcohol on neural development. Results The number of neural precursor cells, neurons and mature glial cells was significantly reduced in the zebrafish embryos following ethanol exposure, and this reduction became more prominent as the ethanol concentration increased. The expression of the early glial marker slc1a3a was down-regulated in the spinal cord but increased in the brain after exposure to increased ethanol concentrations. The expression of the mature glial markers was significantly lowered in response to exposure to increasing ethanol concentrations. Conclusion Ethanol can reduce neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos.