CAJ | 학술논문

目的：研究肌成束蛋白-1（Fascin-1）表达对肝癌细胞 HepG2增殖能力和骨架结构的影响。方法采用 RNA 干扰技术抑制 HepG2中 Fascin-1表达；噻唑蓝（M TT ）法检测细胞增殖；免疫荧光法检测 Fascin-1表达下调后肌动蛋白微丝的改变。结果 RNA 干扰可有效抑制 HepG2中 Fascin-1表达。 Fascin-1蛋白表达下调可抑制细胞增殖，同时引起细胞微丝排列紊乱、断裂、网状结构消失呈絮状，部分细胞收缩。结论 Fascin-1下调引起的肌动蛋白微丝的破坏，一方面可能导致了癌细胞增殖抑制，另一方面可能通过影响细胞运动能力而降低细胞的迁移侵袭能力。
목적：연구기성속단백-1（Fascin-1）표체대간암세포 HepG2증식능력화골가결구적영향。방법채용 RNA 간우기술억제 HepG2중 Fascin-1표체；새서람（M TT ）법검측세포증식；면역형광법검측 Fascin-1표체하조후기동단백미사적개변。결과 RNA 간우가유효억제 HepG2중 Fascin-1표체。 Fascin-1단백표체하조가억제세포증식，동시인기세포미사배렬문란、단렬、망상결구소실정서상，부분세포수축。결론 Fascin-1하조인기적기동단백미사적파배，일방면가능도치료암세포증식억제，령일방면가능통과영향세포운동능력이강저세포적천이침습능력。
Objective To investigate the influence of the expression of Fascin-1 on proliferation and cy-toskeleton of hepatocarcinoma cell line HepG2 .Methods RNA interference (RNAi) was used to inhibit Fascin-1 expression in HepG2 .Cell proliferation was determined by M TT .In addition ,the F-actin of cells was observed with fluorescence microscope .Results Fascin-1 down-regulation could inhibit cells proliferation . HepG2 cy-toskeleton was disorganized as well .Conclusion Expression of Fascin-1 might contribute to the cells prolifera-tions of HepG2 ,which might be related to the cytoskeleton alterations .