CAJ | 학술논문

目的：探讨姜黄素(Curcumin, Cur)在放射线诱导的HT29细胞增殖及凋亡中的作用。方法常规培养HT29细胞，经6 Gy放射线照射和(或) Cur处理24 h后，通过MTT比色法分析空白对照组、6 Gy单独照射组及联合处理组(5μmol/L姜黄素+6 Gy组、10μmol/L姜黄素+6 Gy组、20μmol/L姜黄素+6 Gy组) HT29细胞增殖率,采用流式细胞术Annexin V-FITC/PI双染法分析细胞凋亡率，通过Elisa实验评价细胞中半胱氨酸蛋白酶Caspase-3、Caspase-6和Caspase-9活性。结果 Cur和6 Gy联合处理组的细胞增殖率明显高于空白对照组和6 Gy单独照射组(P<0.05)，细胞凋亡率亦显著高于空白对照组和6 Gy单独照射组(P<0.01)；同时，Cur和6 Gy联合处理组细胞中Caspase-3、Caspase-6和Caspase-9活性亦明显高于对照组和6 Gy单独照射组(P<0.05)。结论 Cur可通过促进细胞凋亡相关因子活性，进而诱导HT29细胞凋亡，抑制其增殖，发挥HT29细胞对放射线的增敏作用，此可为结肠癌放射治疗的临床研究提供新的思路。
목적：탐토강황소(Curcumin, Cur)재방사선유도적HT29세포증식급조망중적작용。방법상규배양HT29세포，경6 Gy방사선조사화(혹) Cur처리24 h후，통과MTT비색법분석공백대조조、6 Gy단독조사조급연합처리조(5μmol/L강황소+6 Gy조、10μmol/L강황소+6 Gy조、20μmol/L강황소+6 Gy조) HT29세포증식솔,채용류식세포술Annexin V-FITC/PI쌍염법분석세포조망솔，통과Elisa실험평개세포중반광안산단백매Caspase-3、Caspase-6화Caspase-9활성。결과 Cur화6 Gy연합처리조적세포증식솔명현고우공백대조조화6 Gy단독조사조(P<0.05)，세포조망솔역현저고우공백대조조화6 Gy단독조사조(P<0.01)；동시，Cur화6 Gy연합처리조세포중Caspase-3、Caspase-6화Caspase-9활성역명현고우대조조화6 Gy단독조사조(P<0.05)。결론 Cur가통과촉진세포조망상관인자활성，진이유도HT29세포조망，억제기증식，발휘HT29세포대방사선적증민작용，차가위결장암방사치료적림상연구제공신적사로。
Objective To explore the effects of curcumin (Cur) on the proliferation and apoptosis of HT29 cells induced by radiotherapy. Methods HT29 cells were routinely cultured and treated with 6 Gy and (or) Cur for 24 h:control group, 6 Gy singly used group,6 Gy combined with Cur group (5μmol/L Cur+6Gy, 10μmol/L Cur+6Gy, 20μmol/L Cur+6Gy). Then MTT assay, flow cytometer, annexin V-FITC/PI double staining and elisa assay were per-formed to evaluate the proliferating ratio, apoptotic ratio and assess the Caspase-3, Caspase-6 and Caspase-9 activi-ties in HT29 cells. Results The cell proliferating ratio of HT29 cells in 6 Gy combined with Cur group was signifi-cantly decreased compared to the corresponding cells in either control or 6 Gy singly used groups (P<0.05), and the apoptotic levels of HT29 cells were significantly increased compared to the corresponding cells in either control or 6Gy singly used groups (P<0.01). Simultaneously, the Caspase-3, Caspase-6 and Caspase-9 activities in the cells in 6 Gy combined with Cur group were increased compared to the corresponding cells in control and 6 Gy singly used groups (P<0.05). Conclusion Cur can increase the sensitivity of HT29 cells in response to 6 Gy through induction of apoptosis-associated factors and increasing apoptosis. Thus, our study may provide a new concern for the clinical treatment of colon carcinoma.