CAJ | 학술논문

目的：研究不同培养浓度CO2对A549活性、合成和分泌肺泡表面活性蛋白C (SP-C)的影响，为允许性高碳酸血症的肺保护机制提供理论依据。方法 A549细胞按不同CO2培养浓度分为A组(5%CO2)、B组(10%CO2)和C组(18%CO2)。细胞培养24 h、36 h、48 h后，采用噻唑蓝(MTT)比色法检测细胞活性；免疫印迹法检测细胞内肺泡表面活性蛋白C前体蛋白(proSP-C)的水平；酶联免疫吸附测定法检测细胞培养上清液中SP-C的浓度。结果(1)与A组比较，B组各观测点的细胞活性均增高；C组的细胞活性则在36 h、48 h降低。(2) B组36 h、48 h细胞培养液中SP-C浓度较A组升高，但是细胞内proSP-C差异无统计学意义。(3)与A组比较，C组24 h培养液中的SP-C浓度已开始降低，但胞内proSP-C在36 h才开始下降。结论(1)一定的CO2培养浓度升高不影响A549的活性，对SP-C的合成和分泌起促进作用；(2)过高的CO2培养浓度可抑制A549的活性、SP-C的合成和分泌。
목적：연구불동배양농도CO2대A549활성、합성화분비폐포표면활성단백C (SP-C)적영향，위윤허성고탄산혈증적폐보호궤제제공이론의거。방법 A549세포안불동CO2배양농도분위A조(5%CO2)、B조(10%CO2)화C조(18%CO2)。세포배양24 h、36 h、48 h후，채용새서람(MTT)비색법검측세포활성；면역인적법검측세포내폐포표면활성단백C전체단백(proSP-C)적수평；매련면역흡부측정법검측세포배양상청액중SP-C적농도。결과(1)여A조비교，B조각관측점적세포활성균증고；C조적세포활성칙재36 h、48 h강저。(2) B조36 h、48 h세포배양액중SP-C농도교A조승고，단시세포내proSP-C차이무통계학의의。(3)여A조비교，C조24 h배양액중적SP-C농도이개시강저，단포내proSP-C재36 h재개시하강。결론(1)일정적CO2배양농도승고불영향A549적활성，대SP-C적합성화분비기촉진작용；(2)과고적CO2배양농도가억제A549적활성、SP-C적합성화분비。
Objective To investigate the effect of different concentrations of carbon dioxide on the synthesis and secretion of surfactant protein C (SP-C) in A549 cells. Methods A549 cells were divided into group A (5%CO2), group B (10%CO2) and group C (18%CO2) according to different concentrations of carbon dioxide. After cul-turing for 24, 36 and 48 hours, the survival state of those cells was estimated by MTT assay, the precursor protein of surfactant protein-C (proSP-C) was measured by western blot, and the concentration of SP-C in the cell culturesuper-natants was measured by ELISA method. Results (1) Compared with group A, group B showed higher activity at 24 h, 36 h, and 48 h, while group C had a lower activity at 36 h and 48 h (MTT assay). (2) SP-C concentration in the culture supernatants of group B was higher than that of group A, however, there was no statistical difference in the expression of proSP-C. (3) Compared with group A, an increasing of SP-C concentration in 24 h culture supernatants and a de-creasing of proSP-C concentration in 36 h culture supernatants in group C were observed. Conclusion (1) 10%CO2 dose not affect the proliferation of A549 cells, but can promote the synthesis and secretion of SP-C in A549 cells;(2) 18%CO2 can inhibite the synthesis and secretion of SP-C as well as limit the proliferation in A549 cells.