CAJ | 학술논문

以转植酸酶玉米10 TPY005、非转植酸酶亲本玉米蠡玉35的花粉分别饲喂家蚕3个龄期，取3龄末期家蚕利用Biolog-Eco法分别从其肠道微生物群落多样性的平均颜色变化率（ AWCD值）、四种碳源利用率、群落代谢功能多样性指数（ Shannon、Simpson和McIntosh指数）及主成分分析四个方面进行指标测定，从而研究转植酸酶玉米对家蚕肠道环境菌群多样性的影响。结果表明：转植酸酶玉米花粉喂食的家蚕与亲本玉米花粉喂食和空白对照两组家蚕相比，上述各指标总体无显著性差异，即转植酸酶玉米对家蚕肠道微生物多样性未造成明显影响。
이전식산매옥미10 TPY005、비전식산매친본옥미려옥35적화분분별사위가잠3개령기，취3령말기가잠이용Biolog-Eco법분별종기장도미생물군락다양성적평균안색변화솔（ AWCD치）、사충탄원이용솔、군락대사공능다양성지수（ Shannon、Simpson화McIntosh지수）급주성분분석사개방면진행지표측정，종이연구전식산매옥미대가잠장도배경균군다양성적영향。결과표명：전식산매옥미화분위식적가잠여친본옥미화분위식화공백대조량조가잠상비，상술각지표총체무현저성차이，즉전식산매옥미대가잠장도미생물다양성미조성명현영향。
The pollen of phytase transgenic maize 10 TPY005 and its non-transgenic parent Liyu 35 were used to feed bombyx for three instars .With the method of Biolog-Eco , the effect of phytase transgenic maize on the diversity of intestinal microbial community in bomdyx were studied by detecting the average well color development (AWCD), utilization ratios of four carbon sources and community metabolism diversity indexes ( Shannon , Simpson and McIntosh indexes ) and principal component analysis .The results showed that there were no significant differences between the bombyx fed by phytase transgenic maize pollen with its non -trans-genic parent pollen and control group .Thereby, there was no obvious effect of phytase transgenic maize on the diversity of intestinal microbial community in bombyx .