CAJ | 학술논문

目的：应用高脂高糖饮食联合链脲佐菌素（STZ）构建巴马小型猪2型糖尿病（T2DM ）模型，探讨STZ对巴马小型猪糖尿病成模的影响，为糖尿病相关研究提供安全稳定的动物模型。方法选用健康雄性巴马小型猪10只，随机分为2组：对照组5例，喂养普通饲料；糖尿病模型组5只，喂养高脂高糖饲料，上述2组共喂养10月。11月初糖尿病模型组腹腔注射STZ 100 mg/kg ，1周后重复1次；对照组腹腔注射等剂量柠檬酸-柠檬酸钠缓冲液。观察2组小型猪体质量、空腹血糖（FPG ）、空腹胰岛素（FINS ）水平，行胰腺组织病理学检查、胰岛B细胞免疫组织化学染色分析，同时计算胰岛素抵抗指数（HOM A-IR）。结果①高脂高糖饲料喂养10月后，模型组小型猪体重、FBG、FINS及 HOMA-IR高于对照组（P＜0.01）；②模型组STZ给药后（12月末试验结束时），与对照组相比，模型组的FPG进一步升高，FINS水平明显下降（P＜0.01）；③胰腺 HE及免疫组织化学染色：成模组镜下胰岛整体形态萎缩，胰岛B细胞数目明显减少。结论采用高脂高糖饮食联合腹腔多次注射STZ可以成功构建巴马小型猪2型糖尿病模型，该方法操作简单、成模率高、稳定性较好。
목적：응용고지고당음식연합련뇨좌균소（STZ）구건파마소형저2형당뇨병（T2DM ）모형，탐토STZ대파마소형저당뇨병성모적영향，위당뇨병상관연구제공안전은정적동물모형。방법선용건강웅성파마소형저10지，수궤분위2조：대조조5례，위양보통사료；당뇨병모형조5지，위양고지고당사료，상술2조공위양10월。11월초당뇨병모형조복강주사STZ 100 mg/kg ，1주후중복1차；대조조복강주사등제량저몽산-저몽산납완충액。관찰2조소형저체질량、공복혈당（FPG ）、공복이도소（FINS ）수평，행이선조직병이학검사、이도B세포면역조직화학염색분석，동시계산이도소저항지수（HOM A-IR）。결과①고지고당사료위양10월후，모형조소형저체중、FBG、FINS급 HOMA-IR고우대조조（P＜0.01）；②모형조STZ급약후（12월말시험결속시），여대조조상비，모형조적FPG진일보승고，FINS수평명현하강（P＜0.01）；③이선 HE급면역조직화학염색：성모조경하이도정체형태위축，이도B세포수목명현감소。결론채용고지고당음식연합복강다차주사STZ가이성공구건파마소형저2형당뇨병모형，해방법조작간단、성모솔고、은정성교호。
Objective To provide a safe and stable animal model of type 2 diabetes (T2DM ) induced by high fat/high sucrose diet and streptozotocin (STZ) ,and investigate the effects of STZ on establishing T 2DM model in Chinese Bama miniature pigs .Methods Ten healthy male Bama miniature pigs were randomly divided into control group (5 ,feeding normal diet) and diabetic group (5 ,feeding high fat and high carbohydrates );the two groups were fed for ten months .At the beginning of eleventh month ,model group was injected with streptozotocin (STZ) 100 mg/kg ,the second intraperitoneal injection of STZ 100mg/kg was 1 week later .The control group was injec-ted with the same amount of sodium citrate solution .In two groups of miniature pig ,the levels of weight ,FPG , FINS and Hcy were detected ,and the parallel pancreatic histopathological examination and pancreas islet B cell im-munohistochemical staining analysis were studied .And the insulin resistance index (HOMA-IR) was calculated . Results ①After feeding high-fat and high-sucrose for ten months ,the serum levels of FPG ,weight ,FINS and HOMA-IR increased significantly in model group compared with control group (P<0.01);②After administration of STZ ,the serum level of FPG increased significantly and the level of FINS was lower in model group compared with control group (P<0.01);③The results of pancreatic HE and immunohistochemical staining :the numbers of islet beta cell were significantly reduced in model group .Conclusion A new T2DM animal model is established by high fat/high sucrose diet and multiple intraperitoneal injection of STZ in Bama miniature pigs .The method has the advantages of simple operation ,high success rate and safety .