CAJ | 학술논문

目的：检测房颤患者维生素K环氧化物还原酶复合物1基因(VKORC1)和细胞色素P450酶2C9基因(CYP2C9)多态性及其对临床使用华法林用量的影响。方法：选择口服华法林抗凝治疗的房颤患者94例,采用PCR扩增和DNA直接测序技术检测患者 VKORC1和CYP2C9基因型,同时记录不同基因型患者华法林日用量,比较不同基因型房颤患者华法林日用量的统计学差异。结果：94例房颤患者检出 VKORC1 AA 型84例, VKORC1 AG型10例,CYP2C9?1/?1型88例,CYP2C9?1/?3型6例。不同 VKORC1、CYP2C9基因型房颤患者华法林用量不同,VKORC1 AA 型并 CYP2C9?1/?1型和 VKORC1 AG型并 CYP2C9?1/?1型患者华法林日用量均高于 VKORC1 AA型并CYP2C9?1/?3型患者,VKORC1 AA 型并 CYP2C9?1/?1型患者华法林用量又高于 VKORC1 AG型并CYP2C9?1/?1型患者。结论：湖北地区房颤患者基因型主要为 VKORC1 AA型与CYP2C9?1/?1型,少量 VKORC1 AG型与CYP2C9?1/?3型。VKORC1、CYP2C9基因型是华法林用量的重要影响因素。
목적：검측방전환자유생소K배양화물환원매복합물1기인(VKORC1)화세포색소P450매2C9기인(CYP2C9)다태성급기대림상사용화법림용량적영향。방법：선택구복화법림항응치료적방전환자94례,채용PCR확증화DNA직접측서기술검측환자 VKORC1화CYP2C9기인형,동시기록불동기인형환자화법림일용량,비교불동기인형방전환자화법림일용량적통계학차이。결과：94례방전환자검출 VKORC1 AA 형84례, VKORC1 AG형10례,CYP2C9?1/?1형88례,CYP2C9?1/?3형6례。불동 VKORC1、CYP2C9기인형방전환자화법림용량불동,VKORC1 AA 형병 CYP2C9?1/?1형화 VKORC1 AG형병 CYP2C9?1/?1형환자화법림일용량균고우 VKORC1 AA형병CYP2C9?1/?3형환자,VKORC1 AA 형병 CYP2C9?1/?1형환자화법림용량우고우 VKORC1 AG형병CYP2C9?1/?1형환자。결론：호북지구방전환자기인형주요위 VKORC1 AA형여CYP2C9?1/?1형,소량 VKORC1 AG형여CYP2C9?1/?3형。VKORC1、CYP2C9기인형시화법림용량적중요영향인소。
Objective:To assess the impact of VKORC1 and CYP2C9 polymorphism on warfarin dosage in pa-tients with atrial fibrillation.Method:Collecting 94 patients of atrial fibrillation using warfarin anticoagulant stability of the standard(INR 2.0-3.0).The genotype of VKORC1 and CYP2C9 were detected by PCR amplification and direct sequencing,and the dosage of warfarin of the patients were recorded and analysed between different geno-types.Results:94 cases were genotyping,84 were VKORC1 AA,10 were VKORC1 AG,88 were CYP2C9?1/?1, 6 were CYP2C9?1/?3.The mean warfarin doses of VKORC1 AA with CYP2C9?1/?1 and VKORC1 AG with CYP2C9?1/?1 were significant more than VKORC1 AA with CYP2C9?1/?3;The mean warfarin doses ofVKORC1 AA with CYP2C9?1/?1 was significant more than VKORC1 AG with CYP2C9?1/?1.Conclusion:The major genotypes of atrial fibrillation related gene were VKORC1 AA and CYP2C9?1/?1 in HuBei.The geno-type of VKORC1 and CYP2C9 were the important influencing factors for warfarin doses of atrial fibrillation.