CAJ | 학술논문

目的：检测分析内皮素-3(EDN-3)基因表达及其启动子甲基化在乳腺癌患者各种临床状态的变化。方法：采用实时定量PCR(RT-PCR)和甲基化PCR(MS-PCR)方法检测54例乳腺癌患者(实验组)及60例体检健康女性(对照组)外周血中 EDN-3 mRNA表达及其启动子甲基化发生率,比较以上两指标变化在乳腺癌患者不同临床状态的统计学差异。结果：实验组 EDN-3 mRNA表达较对照组显著降低,差异有统计学意义(t=-7.84,P<0.01),但这种降低在患者不同年龄、不同肿瘤组织类型和大小、有否区域淋巴结转移及远处转移之间差异均无统计学意义(P>0.05)。实验组 EDN-3启动子甲基化检出率为55.56%(30/54),对照组未检出甲基化,差异有统计学意义(χ2=45.238,P<0.01)；EDN-3启动子甲基化在上述不同临床状态时的差异亦无统计学意义(P>0.05)。结论：EDN-3 mRNA转录下调和启动子甲基化可能与乳腺癌的发生有关,与患者临床病理状态无关。
목적：검측분석내피소-3(EDN-3)기인표체급기계동자갑기화재유선암환자각충림상상태적변화。방법：채용실시정량PCR(RT-PCR)화갑기화PCR(MS-PCR)방법검측54례유선암환자(실험조)급60례체검건강녀성(대조조)외주혈중 EDN-3 mRNA표체급기계동자갑기화발생솔,비교이상량지표변화재유선암환자불동림상상태적통계학차이。결과：실험조 EDN-3 mRNA표체교대조조현저강저,차이유통계학의의(t=-7.84,P<0.01),단저충강저재환자불동년령、불동종류조직류형화대소、유부구역림파결전이급원처전이지간차이균무통계학의의(P>0.05)。실험조 EDN-3계동자갑기화검출솔위55.56%(30/54),대조조미검출갑기화,차이유통계학의의(χ2=45.238,P<0.01)；EDN-3계동자갑기화재상술불동림상상태시적차이역무통계학의의(P>0.05)。결론：EDN-3 mRNA전록하조화계동자갑기화가능여유선암적발생유관,여환자림상병리상태무관。
Objective:To investigate the mRNA expression and the incidence rate of methylation of endothelin-3 (EDN-3)promoter in patients with breast carcinoma in different clinical features.Method:The mRNA expreesi-on and methylation of EDN-3 gene in 54 patients with breast carcinoma (experimental group)and 60 healthy women (control group)were assessed with real-time quantitative polymerase chain reaction (RT-PCR)and methylation-spe-cific polymerase chain reaction (MS-PCR),respectively.The two index above between these gentic features and clinical features were analyzed.Results:The expression of EDN-3 mRNA was significantly lower in experimental group than that of control group (t=-7.84,P<0.01).However there were no significantly differences in different clinical features including the age of patients,tumor tissue type and tumor size,lymph node metastasis and distant metastasis (P>0.05).The detection rate of methylation of EDN-3 promoter was 55.56% (30/54)in experimental group,while the control group was not detected abnormal methylation samples,there were significantly differences between experimental group and control group(χ2=45.238,P<0.01),but there were also no differences among all the clinical fea-tures (P>0.05).Conclusion:The down-regulation of transcription of EDN-3 mRNA and the methylation of EDN-3 pro-moter might be related with the occurrence of breast cancer,but there were no differences among all the clinical features.