陕西科技大学学报（自然科学版）
陝西科技大學學報（自然科學版）
협서과기대학학보（자연과학판）
JOURNAL OF SHAANXI UNIVERSITY OF SCIENCE & TECHNOLOGY
2014年
6期
110-114
,共5页
谷朊粉%酶解%水解度%超声波
穀朊粉%酶解%水解度%超聲波
곡원분%매해%수해도%초성파
vital wheat gluten%enzymolysis%degree of hydrolysis%ultrasonic
以谷朊粉为原料，采用超声辅助碱性蛋白酶酶解制备ACE抑制肽，并对碱性蛋白酶酶解条件进行单因素分析及正交试验设计，确定最优的水解工艺条件．研究结果表明：超声波作用能够提高酶解的水解度和抑制率，同时能够缩短酶解的反应时间．各因素对水解度的影响顺序为：超声功率＞酶浓度＞温度＞时间；最佳酶解工艺条件为：酶浓度为30 m g／g、功率为250 W、时间为60 m in、温度为50℃，此时水解度为14．64％，抑制率为80．37％．
以穀朊粉為原料，採用超聲輔助堿性蛋白酶酶解製備ACE抑製肽，併對堿性蛋白酶酶解條件進行單因素分析及正交試驗設計，確定最優的水解工藝條件．研究結果錶明：超聲波作用能夠提高酶解的水解度和抑製率，同時能夠縮短酶解的反應時間．各因素對水解度的影響順序為：超聲功率＞酶濃度＞溫度＞時間；最佳酶解工藝條件為：酶濃度為30 m g／g、功率為250 W、時間為60 m in、溫度為50℃，此時水解度為14．64％，抑製率為80．37％．
이곡원분위원료，채용초성보조감성단백매매해제비ACE억제태，병대감성단백매매해조건진행단인소분석급정교시험설계，학정최우적수해공예조건．연구결과표명：초성파작용능구제고매해적수해도화억제솔，동시능구축단매해적반응시간．각인소대수해도적영향순서위：초성공솔＞매농도＞온도＞시간；최가매해공예조건위：매농도위30 m g／g、공솔위250 W、시간위60 m in、온도위50℃，차시수해도위14．64％，억제솔위80．37％．
ACE inhibitory peptides was prepared from wheat protein by alkaline hydrolysis methed ,with ultrasonic‐assisted .And the optimum condition was found by single‐factor anal‐ysis and orthogonal test design .The results showed that :ultrasonication could improve the degree of hydrolysis and inhibition rate .At the same time it could short the reaction time of enzyme solution .The order influenced on the degree of hydrolysis rate was as follow :ultra‐sonic power>enzyme concentration> temperature> time .The results showed that the opti‐mum hydrolysis condition was as folloes :The degree of hydrolysis of 14 .64% ,inhibition rate was 80 .37% ,when the enzyme concentration was 30 mg/g at 50 ℃ for 60 minutes and power w as 250 W .
