CAJ | 학술논문

目的：观察神经细胞缺氧/缺糖损伤后线粒体膜电位（MMP）和凋亡的变化情况及阿司匹林的保护作用。方法用体外培养7 d的Wistar大鼠皮质神经细胞，随机分为正常对照组、缺氧/缺糖模型组、缺氧/缺糖加100μmol/L浓度阿司匹林组。缺氧/缺糖2 h处理后，四甲基偶氮唑盐（MTT）比色分析法检测神经元线粒体活性；流式细胞术检测神经元线粒体膜电位、双染法检测不同组神经元的凋亡情况。结果缺氧/缺糖损伤2h后，模型组的线粒体活性和MMP 水平较对照组显著降低（P<0.01）。细胞凋亡百分率均较对照组显著升高（P<0.01）。而阿司匹林组能显著提高神经元线粒体活性、膜电位，降低细胞凋亡的百分率。结论阿司匹林可抑制缺氧/缺糖损伤所致的神经元线粒体活性和膜电位的降低、稳定线粒体膜电位，抑制神经元凋亡，起到神经元保护作用。
목적：관찰신경세포결양/결당손상후선립체막전위（MMP）화조망적변화정황급아사필림적보호작용。방법용체외배양7 d적Wistar대서피질신경세포，수궤분위정상대조조、결양/결당모형조、결양/결당가100μmol/L농도아사필림조。결양/결당2 h처리후，사갑기우담서염（MTT）비색분석법검측신경원선립체활성；류식세포술검측신경원선립체막전위、쌍염법검측불동조신경원적조망정황。결과결양/결당손상2h후，모형조적선립체활성화MMP 수평교대조조현저강저（P<0.01）。세포조망백분솔균교대조조현저승고（P<0.01）。이아사필림조능현저제고신경원선립체활성、막전위，강저세포조망적백분솔。결론아사필림가억제결양/결당손상소치적신경원선립체활성화막전위적강저、은정선립체막전위，억제신경원조망，기도신경원보호작용。
Objective To investigate the neuroprotective effects of aspirin on mitochondrial membrane potential (MMP) and apoptosis following oxygen/glucose deprivation (OGD)-induced injury in neurons. Methods Cultured cor-tex neurons harvested from Wistar rats at day 7 were randomly assigned to normal control group, OGD group, and OGD plus aspirin treatment (100 μmol/L) group, respectively. Following OGD for 2 hours, mitochondrion activity was measured based on the methyl thiazolyl tetrazolium (MTT) test. The MMP was measured by flow cytometry. Neuronal necrosis were assayed with annexin-FITC and PI double immunofluorescence microscopy. Results Following OGD for 2 hours, the MMP and mitochondrion activity were consistently reduced and the percentage of neuronal apoptosis increased (all P<0.01). Aspirin significantly restored mitochondrion activity, decreased the percentage of neuron apop-tosis, and increased the MMP. Conclusion Aspirin significantly abrogates the decline of MMP, restores mitochondri-on activity, stabilizes the MMP and inhibits neuronal apoptosis following OGD induced injury, which represents the neuroprotective effects.