CAJ | 학술논문

试验选取中性蛋白酶对扇贝裙边进行水解。在单因素试验的基础上,采用响应面法(RSM)考察主要因素对水解度(DH)的影响,并建立回归模型。试验结果表明中性蛋白酶的最佳酶解条件：加酶量23154 U/g 干物质,底物浓度为7%,温度49.1℃,pH 7.8,酶解时间为4 h,在此条件下测得的水解率为50.420%,与预测值的相对误差为1.087%,预测模型与实际情况拟合较好。
시험선취중성단백매대선패군변진행수해。재단인소시험적기출상,채용향응면법(RSM)고찰주요인소대수해도(DH)적영향,병건립회귀모형。시험결과표명중성단백매적최가매해조건：가매량23154 U/g 간물질,저물농도위7%,온도49.1℃,pH 7.8,매해시간위4 h,재차조건하측득적수해솔위50.420%,여예측치적상대오차위1.087%,예측모형여실제정황의합교호。
Scallop skirt is hydrolyzed with neutral protease.Based on the single factor experiment,the response surface methodology (RSM)is employed to investigate the main influence factors on the degree of hydrolysis (DH)to establish a regression model.The conditions of enzymic hydrolysis for scallop skirt are optimized by RSM as follows:substrate concentration of 7%,concentration of enzyme of 23154 (U/g dry matter),hydrolysis temperature of 49.1 ℃,pH 7.8,hydrolysis time of 4 h,the optimized DH could reach 50.420%.The experimental values agree with those predicted from the regression model within a relative error of 1.087%,indicating a good fitness.