CAJ | 학술논문

目的：：研究7周大负荷游泳运动对大鼠空间学习记忆及海马神经粘附分子( NCAM)的影响。方法：雄性SD大鼠40只,随机分为空白对照组( C组)、空白+水迷宫组( CM组)、大负荷运动组( HL组)和大负荷运动+水迷宫组( HLM组)4组,每组10只。C组和CM组常规饲养,不加干预；HL组和HLM组进行7周大负荷游泳运动。7周后,C组和HL组断头取海马组织,采用Real-time PCR和Western blot检测NCAM mRNA和蛋白表达；7周后,应用Morris水迷宫检测CM组和HLM组大鼠定位航行和空间探索次数。结果：(1)实验期间,HL组大鼠体重第5-7周末均非常显著低于C组(P<0.01)；HLM组大鼠体重第4-7周末均非常显著低于CM组(P<0.01)。(2)Morris水迷宫平均逃避潜伏期,两组在前3天的空间探索逃避潜伏期与后5天的学习记忆均有非常显著性差异(P<0.01),CM组第2~10天平均逃避潜伏期均显著性短于第1天(P<0.01),第3-10天平均逃避潜伏期均显著性短于第2天(P<0.05)；HLM组第2~10天平均逃避潜伏期均显著性低于第1天(P<0.01),第4-10天平均逃避潜伏期均显著性短于第2天(P<0.05)。 Morris水迷宫空间探索实验,HLM组平均穿越次数多于CM组(P>0.05)。(3)HL组NCAM基因和蛋白表达均高于C组(P<0.05)。结论：7周大负荷运动后虽然大鼠逃避潜伏期缩短了,空间探索穿越次数有所增加,但随负荷增加,大鼠表现就越不稳定,可能影响或阻碍大鼠学习记忆的形成；长期大负荷运动提高了大鼠海马NCAM基因和蛋白表达,提示其机制可能是参与了损伤后神经的修复。
목적：：연구7주대부하유영운동대대서공간학습기억급해마신경점부분자( NCAM)적영향。방법：웅성SD대서40지,수궤분위공백대조조( C조)、공백+수미궁조( CM조)、대부하운동조( HL조)화대부하운동+수미궁조( HLM조)4조,매조10지。C조화CM조상규사양,불가간예；HL조화HLM조진행7주대부하유영운동。7주후,C조화HL조단두취해마조직,채용Real-time PCR화Western blot검측NCAM mRNA화단백표체；7주후,응용Morris수미궁검측CM조화HLM조대서정위항행화공간탐색차수。결과：(1)실험기간,HL조대서체중제5-7주말균비상현저저우C조(P<0.01)；HLM조대서체중제4-7주말균비상현저저우CM조(P<0.01)。(2)Morris수미궁평균도피잠복기,량조재전3천적공간탐색도피잠복기여후5천적학습기억균유비상현저성차이(P<0.01),CM조제2~10천평균도피잠복기균현저성단우제1천(P<0.01),제3-10천평균도피잠복기균현저성단우제2천(P<0.05)；HLM조제2~10천평균도피잠복기균현저성저우제1천(P<0.01),제4-10천평균도피잠복기균현저성단우제2천(P<0.05)。 Morris수미궁공간탐색실험,HLM조평균천월차수다우CM조(P>0.05)。(3)HL조NCAM기인화단백표체균고우C조(P<0.05)。결론：7주대부하운동후수연대서도피잠복기축단료,공간탐색천월차수유소증가,단수부하증가,대서표현취월불은정,가능영향혹조애대서학습기억적형성；장기대부하운동제고료대서해마NCAM기인화단백표체,제시기궤제가능시삼여료손상후신경적수복。
Objective:The purpose is to investigate the effect of long-term heavy load swimming exercise on the spatial learning and memorizing abilities and the expression of neural cell adhesion molecule( NCAM) in hippocampus of SD rats. Methods:40 male rats were randomly divided into 4 groups as follows( n=10 in each group): control group ( C) , control group and Morris maze( CM) , and both were conventionally bred without intervening;7 weeks of moderate swimming exercise were given to heavy load exercise group(HL) and Morris maze group (HLM). After 7 weeks, the brain of the C and HL group’ s rats were taken out to test the mRNA and protein of NCAM through real-time PCR and Western Blotting. The navigation and space exploration ability of CM and HLM were tested through Morris water maze. Results:1. In the period of swimming, the weight of HL group rats was significantly lower than that of C group at 5, 6 and 7 weekends(p<0. 01);the weight of HLM group rats was significantly lower than that of CM group at 4, 5, 6 and 7 weekends (P < 0. 05,P < 0. 01). 2. After Morris maze training, the early 3 days evasion incubation period of the two groups and the last 5 days ability of learning and memory had a significantly difference(p<0. 01). The average escape latency of CM group from 2 to 10 days was significantly lower than the first day ( p<0 . 01 ) , and it became significantly lower from 3rd to 10th days than the second day(p<0. 05,p<0. 01); the average escape latency of HLM group from the 2nd to 10th days was significantly lower than the first day(p<0. 01),and it was significantly lower from 4th to 10th days than the second day(p<0. 05,p<0. 01). After Morris maze training, the average traversing times of HLM group were more than those in CM group ( p>0 . 05 ) . 3 . The expression of NCAM gene and protein of the HL group rats were significantly more active than the C group rats(P<0. 05). Conclusion:Although 7-weeks of long-term heavy load ex-ercise can shorten the evasion incubation period of locating swimming and enhance the passing frequency of spatial explo-ring of rats, within the increase of load and the accumulation of fatigue, the rats behaved more unstably and it will affect the formation of learning and memory in rats;long-term heavy load exercise is conducive to the promotion of NCAM ex-pression in rats hippocampus, which shows that the mechanism may be associated with the repairmen of injured nerves.