北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2014年
12期
104-104,105
,共2页
三七总皂苷%脑缺血%再灌注损伤%TGF-β1
三七總皂苷%腦缺血%再灌註損傷%TGF-β1
삼칠총조감%뇌결혈%재관주손상%TGF-β1
Panax notoginseng saponins%Cerebral ischemia%Reperfusion injury%TGF-β1
目的：观察三七总皂苷（PNS）对局灶性脑缺血再灌注后大鼠脑组织TGF-β1表达的影响，探讨PNS的脑保护机制。方法：采用线栓法制作大鼠大脑中动脉栓塞缺血再灌注损伤模型，将30只成年雄性Sprague-Darley大鼠随机分为假手术组、缺血再灌注组和PNS治疗组，每组10只，各组分别于再灌注24h后做神经功能缺陷评估，并断头取脑，用免疫组织化学法和半定量RT-PCR法检测各组缺血区脑组织TGF-β1表达的变化。结果：与缺血再灌注组相比，PNS治疗组术后大鼠神经功能缺陷评分显著降低，脑组织中TGF-β1的表达水平明显增高。结论：三七总皂苷对脑缺血再灌注大鼠有保护作用，其作用机制可能与促进TGF-β1的表达有关。
目的：觀察三七總皂苷（PNS）對跼竈性腦缺血再灌註後大鼠腦組織TGF-β1錶達的影響，探討PNS的腦保護機製。方法：採用線栓法製作大鼠大腦中動脈栓塞缺血再灌註損傷模型，將30隻成年雄性Sprague-Darley大鼠隨機分為假手術組、缺血再灌註組和PNS治療組，每組10隻，各組分彆于再灌註24h後做神經功能缺陷評估，併斷頭取腦，用免疫組織化學法和半定量RT-PCR法檢測各組缺血區腦組織TGF-β1錶達的變化。結果：與缺血再灌註組相比，PNS治療組術後大鼠神經功能缺陷評分顯著降低，腦組織中TGF-β1的錶達水平明顯增高。結論：三七總皂苷對腦缺血再灌註大鼠有保護作用，其作用機製可能與促進TGF-β1的錶達有關。
목적：관찰삼칠총조감（PNS）대국조성뇌결혈재관주후대서뇌조직TGF-β1표체적영향，탐토PNS적뇌보호궤제。방법：채용선전법제작대서대뇌중동맥전새결혈재관주손상모형，장30지성년웅성Sprague-Darley대서수궤분위가수술조、결혈재관주조화PNS치료조，매조10지，각조분별우재관주24h후주신경공능결함평고，병단두취뇌，용면역조직화학법화반정량RT-PCR법검측각조결혈구뇌조직TGF-β1표체적변화。결과：여결혈재관주조상비，PNS치료조술후대서신경공능결함평분현저강저，뇌조직중TGF-β1적표체수평명현증고。결론：삼칠총조감대뇌결혈재관주대서유보호작용，기작용궤제가능여촉진TGF-β1적표체유관。
Objective:To investigate the effect of panax notoginseng saponins(PNS)on the expression of TGF-β1 in cerebral cortex during focal cerebral ischemia-reperfusion injury in rats. Methods:30 male adult Sprague-Dawley rats were randomly divided into 3 groups:sham-operation group,ischemia-reperfusion group and PNS treatment group. At 24h after reperfusion, scores of neurological deficit were estimated. The rats were killed to detect the expression of TGF-β1 in ischemic region by immunohistochemistry and RT-PCR. Results:Compared with the ischemia-reperfusion group, the scores of neurological deficit was decreased and the expression of TGF-β1 was increased significantly in PNS treatment group. Conclusion:The panax notoginseng saponins can lighten the neurons injury after focal cerebral ischemia-reperfusion injury in rats partly by stimulating the expression of TGF-β1 in the brain.
