CAJ | 학술논문

目的：分析抗菌肽人β防御素3（hBD3）对耐甲氧西林金黄色葡萄球菌 N315株的抑制作用。方法合成抗菌肽 hBD3，分别通过最低抑菌浓度（MIC）检测、直接杀菌试验、重要功能基因检测分析其对 N315株的直接抑制作用，并观察对抗生素 MIC 的影响。结果hBD3对 N315株的 MIC 为8μg/mL；在浓度达到2μg/mL 时即有明显杀菌作用。hBD3上调 N315株的 agr 基因表达，下调 icaA 基因表达。在联用5μg/mL 的 hBD3后，青霉素、头孢拉定、万古霉素、头孢呋辛、氯霉素的 MIC 值均有降低。结论抗菌肽 hBD3对 MRSA N315株有显著的抑制作用，有望将其与抗生素联用可抑制抗生素耐药性的扩散。
목적：분석항균태인β방어소3（hBD3）대내갑양서림금황색포도구균 N315주적억제작용。방법합성항균태 hBD3，분별통과최저억균농도（MIC）검측、직접살균시험、중요공능기인검측분석기대 N315주적직접억제작용，병관찰대항생소 MIC 적영향。결과hBD3대 N315주적 MIC 위8μg/mL；재농도체도2μg/mL 시즉유명현살균작용。hBD3상조 N315주적 agr 기인표체，하조 icaA 기인표체。재련용5μg/mL 적 hBD3후，청매소、두포랍정、만고매소、두포부신、록매소적 MIC 치균유강저。결론항균태 hBD3대 MRSA N315주유현저적억제작용，유망장기여항생소련용가억제항생소내약성적확산。
Objective To analyze the inhibition effect of antimicrobial peptide human βdefensin 3 (hBD3 )against methicillin resistant Staphylococcus aureus (MRSA)N31 5.Methods Antimicrobial peptide hBD3 was synthesized.The direct inhibition on N31 5 was detected by the MIC (minimal inhibitory concentration,MIC)test,antibacterial activity test and the analysis on some important functional genes.The peptide was combined with penicillin,tetracycline,cephradine, rifampin,vancomycin,cefuroxime and chloramphenicol to evaluate the effects on antibiotic MIC values.Results The MIC of hBD3 against N31 5 was 8 μg/mL and the bactericidal activity was observed while the hBD3 concentration reached 2 μg/mL.The peptide hBD3 could improve the expression of agr and reduce the expression of icaA.With 5 μg/mL peptide hBD3,the MIC values against N31 5 of penicillin,cephradine,vancomycin,cefuroxime and chloramphenicol were dropped down.Conclusion There is considerable bactericidal activity of antimicrobial peptide hBD3 against MRSA N31 5. Antibiotic peptide hBD3 could be used to prevent the spread of antibiotic resistant bacteria in the future.