CAJ | 학술논문

采用超声波辅助萃取法提取文冠果种皮中的总皂苷。考察了乙醇体积分数、超声波功率、料液比、提取时间和提取温度对总皂苷提取率的影响,在单因素试验的基础上选取对总皂苷提取率影响显著的料液比、乙醇体积分数和超声波功率,利用Box-Behnken设计原理,以总皂苷提取率为响应值进行响应面试验,建立了回归方程,得到了优化的提取条件(料液比1∶30,乙醇体积分数73%,超声波功率220 W)。在提取温度50℃、提取时间90 min及优化的提取条件下,文冠果种皮总皂苷提取率达0.30%。
채용초성파보조췌취법제취문관과충피중적총조감。고찰료을순체적분수、초성파공솔、료액비、제취시간화제취온도대총조감제취솔적영향,재단인소시험적기출상선취대총조감제취솔영향현저적료액비、을순체적분수화초성파공솔,이용Box-Behnken설계원리,이총조감제취솔위향응치진행향응면시험,건립료회귀방정,득도료우화적제취조건(료액비1∶30,을순체적분수73%,초성파공솔220 W)。재제취온도50℃、제취시간90 min급우화적제취조건하,문관과충피총조감제취솔체0.30%。
Total saponins were extracted from the seed husk of Xanthoceras sorbifolia Bunge by ultrasound-assisted extraction method. The effects of volume fraction of ethanol, ultrasonic power, ratio of solid to liquid, extraction time and extraction temperature on the extraction rate of total saponins were investiga-ted. Based on single factor experiment, with volume fraction of ethanol, ultrasonic power, ratio of solid to liquid those most significantly affected the extraction rate of total saponins as influencing factors, extrac-tion rate of total saponins as response value, the response surface experiment was conducted by the Box-Behnken design and the regression model was established. The results showed that the optimal extraction conditions were obtained as follows: volume fraction of ethanol 73%, ultrasonic power 220 W, ratio of solid to liquid 1∶30. Under the optimal conditions, the extraction rate of total saponins reached 0. 30%when extraction temperature and extraction time were 50℃ and 90 min respectively.