中国油脂
中國油脂
중국유지
CHINA OILS AND FATS
2014年
11期
69-73
,共5页
茶皂素%大孔树脂%吸附%解吸%纯化
茶皂素%大孔樹脂%吸附%解吸%純化
다조소%대공수지%흡부%해흡%순화
tea saponin%macroporous resin%adsorption%desorption%purification
采用大孔树脂对茶皂素粗品进行分离纯化。以茶皂素得率为指标,采用响应面法优化洗脱条件。通过考察不同极性的大孔树脂D-101、AB-8、S-8、X-5、HPD-100的吸附与解吸性能,从中筛选出具有代表性的S-8大孔树脂,并对其吸附和解吸能力进行综合分析。确定S-8大孔树脂纯化茶皂素最佳工艺条件为：S-8大孔树脂装柱量100 g,质量浓度为0.703 mg/mL的茶皂素溶液110 mL,上样流速3.03 mL/min,洗脱剂乙醇体积分数90%,乙醇流速3.03 mL/min,乙醇用量100 mL。在最佳条件下,对含量为47.38%的茶皂素粗品进行分离纯化,最终茶皂素得率为81.74%,回收率为78.29%,含量为85.36%。
採用大孔樹脂對茶皂素粗品進行分離純化。以茶皂素得率為指標,採用響應麵法優化洗脫條件。通過攷察不同極性的大孔樹脂D-101、AB-8、S-8、X-5、HPD-100的吸附與解吸性能,從中篩選齣具有代錶性的S-8大孔樹脂,併對其吸附和解吸能力進行綜閤分析。確定S-8大孔樹脂純化茶皂素最佳工藝條件為：S-8大孔樹脂裝柱量100 g,質量濃度為0.703 mg/mL的茶皂素溶液110 mL,上樣流速3.03 mL/min,洗脫劑乙醇體積分數90%,乙醇流速3.03 mL/min,乙醇用量100 mL。在最佳條件下,對含量為47.38%的茶皂素粗品進行分離純化,最終茶皂素得率為81.74%,迴收率為78.29%,含量為85.36%。
채용대공수지대다조소조품진행분리순화。이다조소득솔위지표,채용향응면법우화세탈조건。통과고찰불동겁성적대공수지D-101、AB-8、S-8、X-5、HPD-100적흡부여해흡성능,종중사선출구유대표성적S-8대공수지,병대기흡부화해흡능력진행종합분석。학정S-8대공수지순화다조소최가공예조건위：S-8대공수지장주량100 g,질량농도위0.703 mg/mL적다조소용액110 mL,상양류속3.03 mL/min,세탈제을순체적분수90%,을순류속3.03 mL/min,을순용량100 mL。재최가조건하,대함량위47.38%적다조소조품진행분리순화,최종다조소득솔위81.74%,회수솔위78.29%,함량위85.36%。
The crude tea saponin was separated and purified by macroporous resin. Using the yield of tea saponin as indicator, the elution conditions were optimized by response surface methodology. S -8 macroporous resin was screened out by studying the adsorption and desorption properties of macroporous resins D-101 , AB-8 , S-8 , X-5 and HPD-100 . The optimal adsorption conditions were obtained as follows: dosage of S - 8 macroporous resin 100 g, mass concentration of tea sapoin solution 0. 703 mg/mL, loading volume 110 mL and flow rate of sample 3. 03 mL/min, volume fraction of ethanol (eluent)90%, flow rate 3. 03 mL/min, dosage of ethanol 100 mL. After 47. 38% of tea saponin was separated and purified under the optimal conditions, the yield, recovery rate and content of tea saponin were 81. 74%, 78. 29% and 85. 36%,respectively.
