CAJ | 학술논문

目的：对从长期种植枸杞的土壤中分离筛选出的一株可高效降解农药啶虫脒的细菌 AC04进行急性毒性的安全性评价。方法将提取的AC04菌液对小鼠进行经口灌胃急性毒性实验；将表面喷洒菌悬液的枸杞对大鼠进行30 d喂养实验。结果经口急性毒性实验中实验小鼠行为、进食均正常，未出现中毒及死亡情况。观察期结束后，解剖动物主要脏器、器官，均未见明显异常改变。大鼠30d喂养实验中，实验动物的体征外观、行为、体重、血象等各项指标均正常，无明显中毒症状或不良反应。结论该菌株及其代谢产物对试验鼠未见明显毒性。
목적：대종장기충식구기적토양중분리사선출적일주가고효강해농약정충미적세균 AC04진행급성독성적안전성평개。방법장제취적AC04균액대소서진행경구관위급성독성실험；장표면분쇄균현액적구기대대서진행30 d위양실험。결과경구급성독성실험중실험소서행위、진식균정상，미출현중독급사망정황。관찰기결속후，해부동물주요장기、기관，균미견명현이상개변。대서30d위양실험중，실험동물적체정외관、행위、체중、혈상등각항지표균정상，무명현중독증상혹불량반응。결론해균주급기대사산물대시험서미견명현독성。
Objective To evaluate a new screened strain of bacteria AC04 found in the soil of long-term planting of Chinese wolfberry which can efficiently degrade the pesticide acetamiprid using rodent acute toxic-ity models. MethodsThe extracted fluid of bacteria was given to mice for acute toxicity experiment by ig. The rats were fed with wolfberry sprayed by the bacterial suspension for 30 d. Results The acute oral tox-icity tests showed the tested mice behaving and eating normal, without poisoning and death. At the end of the observation, the mice were dissected and the major organs showed no abnormalities. In the experiments of rats fed for 30 d, the appearance of experimental rats, as well as the behavior, body weight, and blood indicators showed normal, without obvious symptoms or adverse reactions. Conclusion The research showed that this strain of bacteria was harmless to the experimental animals.