CAJ | 학술논문

目的：利用超高效液相色谱建立一种麦卢卡蜂蜜中丙酮醛的快速检测方法。方法采用乙酸钠缓冲溶液直接提取蜂蜜中的丙酮醛,提取液与2,4-二硝基苯肼溶液室温避光衍生20 h后,经C18色谱柱分离后采用二极管阵列检测器(PDA)检测,外标法定量。结果丙酮醛的检出限为10.0 mg/kg,定量限为25.0 mg/kg,在1.0~60 mg/L之间线性关系良好,相关系数为0.9982,在蜂蜜中添加25.0、500、1000三个水平的回收率在93.9%~97.8%之间,相对标准偏差在1.8%~5.4%之间。结论该分析方能够满足麦卢卡蜂蜜中丙酮醛的快速测定要求。
목적：이용초고효액상색보건립일충맥로잡봉밀중병동철적쾌속검측방법。방법채용을산납완충용액직접제취봉밀중적병동철,제취액여2,4-이초기분정용액실온피광연생20 h후,경C18색보주분리후채용이겁관진렬검측기(PDA)검측,외표법정량。결과병동철적검출한위10.0 mg/kg,정량한위25.0 mg/kg,재1.0~60 mg/L지간선성관계량호,상관계수위0.9982,재봉밀중첨가25.0、500、1000삼개수평적회수솔재93.9%~97.8%지간,상대표준편차재1.8%~5.4%지간。결론해분석방능구만족맥로잡봉밀중병동철적쾌속측정요구。
Objective To establish a fast detection method of methylglyoxal (MGO) in Manuka honey by ultra performance liquid chromatography (UPLC).MethodsMGO in honey was extracted by sodium acetate buffer solution, extract solution derivatized 20 h with 2,4-dinitrophenylhydrazine solution, and detected by PDA detector after separated by C18 chromatographic column, quantified by external standard calibration me-thod.Results The detection limit of MGO was 10.0 mg/kg, limit of quantification was 25.0 mg/kg. The me-thod showed a good linear range of detection between 1.0~60 mg/L mg/L (r2=0.9982). The recoveries of MGO at three spiked levels (25.0, 500, and 1000 mg/kg) were between 93.9% ~ 97.8 %, RSD between 1.8% ~ 5.4%. Conclusion The method is suitable for fast analysis of MGO in Manuka honey.