CAJ | 학술논문

目的：研究转基因大豆对硒的富集作用以及富集前后转基因大豆中硒的形态分布和形态转化。方法采用电感耦合等离子体质谱分析转基因大豆富集前后硒的总量,采用蛋白酶提取,高效液相色谱-电感耦合等离子体质谱(high performance liquid chromatography-inductively coupled plasma-mass spectrometry, HPLC-ICP-MS)联用技术分析转基因大豆中的硒酸盐(selenate, SeVI)、亚硒酸盐(selenite, SeIV)、硒代蛋氨酸(selenomethionine, SeMet)和硒代胱氨酸(selenocystine, SeCys)等几种硒化合物。盆栽种植试验研究转基因大豆对硒的富集作用。通过对照试验,考察大豆植株不同部位总硒含量变化和形态分布的转化情况。结果转基因大豆对硒有较好的富集作用,吸收的亚硒酸盐在大豆植株中部分转化成了硒代蛋氨酸和硒代胱氨酸。结论了解了转基因大豆的形态分布和转化情况,可以更好地评估转基因大豆的食用安全风险并进行生物利用开发。
목적：연구전기인대두대서적부집작용이급부집전후전기인대두중서적형태분포화형태전화。방법채용전감우합등리자체질보분석전기인대두부집전후서적총량,채용단백매제취,고효액상색보-전감우합등리자체질보(high performance liquid chromatography-inductively coupled plasma-mass spectrometry, HPLC-ICP-MS)련용기술분석전기인대두중적서산염(selenate, SeVI)、아서산염(selenite, SeIV)、서대단안산(selenomethionine, SeMet)화서대광안산(selenocystine, SeCys)등궤충서화합물。분재충식시험연구전기인대두대서적부집작용。통과대조시험,고찰대두식주불동부위총서함량변화화형태분포적전화정황。결과전기인대두대서유교호적부집작용,흡수적아서산염재대두식주중부분전화성료서대단안산화서대광안산。결론료해료전기인대두적형태분포화전화정황,가이경호지평고전기인대두적식용안전풍험병진행생물이용개발。
Objective Tostudy the selenium enrichment of genetically modified soybean, selenium com-pound species distribution and transformation before and after enrichment.MethodsThe total selenium con-tent in genetically modified soybean were detected by inductively coupled plasma-mass spectrometry. Ex-tracted with protease, selenium species of selenate [Se(VI)], selenite [Se(IV)], selenomethionine (SeMet) and selenocystine (SeCys) in genetically modified soybean were detected using high performance liquid chromato-graphy-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The changes of total selenium content in different parts of the soybean plant and distribution of transformation of selenium species based on the con-trast test, which were made by potted planting, were also studied.Results The genetically modified soybean had a better enrichment on selenium, with the selenite uptaken by soybean plant partly being converted into se-lenomethionine and selenocystine. ConclusionThe analysis of distribution and transformation of selenium and its species will push forward the evaluation of food safety risks and the development of biological utiliza-tion of genetically modified soybean.