CAJ | 학술논문

目的通过气道内滴注腺病毒转染肝细胞生长因子(adenovirus hepatocyte growth factor,Ad-HGF)干预野百合碱(monocrotaline,MCT)诱导的肺动脉高压(pulmonary hypertension,PAH)模型大鼠,观察Ad-HGF干预对平均肺动脉压(mean pulmonary artery pressure,mPAP)等指标的影响.方法选用健康雄性SD大鼠40只,随机[采用随机单位(区)组设计方法]分为4组:正常对照组(normal,NOR组)10只,MCT诱导PAH组(PAH组)10只、单次和重复肝细胞生长因子(hepatocyte growth factor,HGF)干预治疗组(HGF和THGF组)各10只.NOR组和PAH组:气道内滴注0.2 mL磷酸盐缓冲液;HGF组和THGF组:气道内滴注0.2 mL Ad-HGF 1次和一周后重复一次共2次.再饲养两周后,测定各组大鼠的肺动脉压,计算右心室肥厚指数;苏木素伊红染色观察肺动脉管壁等;酶联免疫吸附(ELISA)法测定肺组织匀浆中HGF浓度.结果与PAH组大鼠比较,HGF组和THGF组大鼠的mPAP、右心室肥厚指数、肺动脉管壁指数和面积指数明显降低,肺苏木素伊红染色肺小动脉管壁厚度减少,管腔面积增大,血管周围炎症细胞浸润减轻,肺组织匀浆HGF浓度明显升高,差异有统计学意义(P＜0.05),且低于NOR组水平.结论经气道内滴注转染Ad-HGF,能明显降低但不能完全逆转MCT诱导PAH大鼠的mPAP,减少肺小动脉管壁厚度,减轻右心室肥厚程度,从而达到延缓PAH进程的作用.
목적 통과기도내적주선병독전염간세포생장인자(adenovirus hepatocyte growth factor,Ad-HGF)간예야백합감(monocrotaline,MCT)유도적폐동맥고압(pulmonary hypertension,PAH)모형대서,관찰Ad-HGF간예대평균폐동맥압(mean pulmonary artery pressure,mPAP)등지표적영향.방법 선용건강웅성SD대서40지,수궤[채용수궤단위(구)조설계방법]분위4조:정상대조조(normal,NOR조)10지,MCT유도PAH조(PAH조)10지、단차화중복간세포생장인자(hepatocyte growth factor,HGF)간예치료조(HGF화THGF조)각10지.NOR조화PAH조:기도내적주0.2 mL린산염완충액;HGF조화THGF조:기도내적주0.2 mL Ad-HGF 1차화일주후중복일차공2차.재사양량주후,측정각조대서적폐동맥압,계산우심실비후지수;소목소이홍염색관찰폐동맥관벽등;매련면역흡부(ELISA)법측정폐조직균장중HGF농도.결과 여PAH조대서비교,HGF조화THGF조대서적mPAP、우심실비후지수、폐동맥관벽지수화면적지수명현강저,폐소목소이홍염색폐소동맥관벽후도감소,관강면적증대,혈관주위염증세포침윤감경,폐조직균장HGF농도명현승고,차이유통계학의의(P＜0.05),차저우NOR조수평.결론 경기도내적주전염Ad-HGF,능명현강저단불능완전역전MCT유도PAH대서적mPAP,감소폐소동맥관벽후도,감경우심실비후정도,종이체도연완PAH진정적작용.