CAJ | 학술논문

目的：探讨miR-184在氧糖剥夺（OGD）诱导SK-N-SH细胞缺血缺氧损伤中的作用及其对抗凋亡基因AKT2的调节。方法：应用RT-PCR检测miR-184在SK-N-SH细胞OGD后的表达情况，并瞬时转染miR-184的类似物、抑制物及其阴性对照，分别行RT-PCR检测miR-184和AKT2的表达，MTT染色检测细胞存活率。结果：与正常细胞相比，miR-184在OGD诱导的缺血损伤模型中表达下调（P＜0．05）；且过表达或抑制miR-184可显著改变AKT2的表达，影响缺血损伤细胞的存活率（P＜0．05）；而在非OGD作用下，miR-184对SK-N-SH细胞的活力无显著影响。结论：miR-184通过负性调节AKT2的表达水平，在OGD诱导的缺血损伤中发挥重要作用。
목적：탐토miR-184재양당박탈（OGD）유도SK-N-SH세포결혈결양손상중적작용급기대항조망기인AKT2적조절。방법：응용RT-PCR검측miR-184재SK-N-SH세포OGD후적표체정황，병순시전염miR-184적유사물、억제물급기음성대조，분별행RT-PCR검측miR-184화AKT2적표체，MTT염색검측세포존활솔。결과：여정상세포상비，miR-184재OGD유도적결혈손상모형중표체하조（P＜0．05）；차과표체혹억제miR-184가현저개변AKT2적표체，영향결혈손상세포적존활솔（P＜0．05）；이재비OGD작용하，miR-184대SK-N-SH세포적활력무현저영향。결론：miR-184통과부성조절AKT2적표체수평，재OGD유도적결혈손상중발휘중요작용。
Objective To explore the role of miR-184 in Oxygen-Glucose-Deprivation (OGD) induced SK-N-SH cell ischemic injury and its regulation on AKT2 level. Method We used a combination of oxygen and glucose deprivation to imitate ischemic conditions in vivo. MiR-184 mimic and inhibitor were transfected into SK-N-SH cell to alter miR-184 levels. The expression of miR-184 and AKT2 were determined by using Real-time PCR. The extent of SK-N-SH cell survival rate was assessed by thiazolyl blue tetrazolium bromide (MTT) assay. Result Here, we observed that miR-184 was significantly inhibited in SK-N-SH cell after OGD (P<0.05). The changes of miR-184 level altered the expression of AKT2 mRNA. In addition, alteration of miR-184expressionsignificantly affected cell survival rate after OGD. Conclusion miR-184 plays an important role in ischemic injury through negatively regulating AKT2 level, which may provide a potential therapeutic target for ischemic stroke in miRNA levels.