实用医学杂志
實用醫學雜誌
실용의학잡지
THE JOURNAL OF PRACTICAL MEDICINE
2014年
20期
3213-3215
,共3页
梁悦%侯长春%黄宏%邬丽红%陈一强
樑悅%侯長春%黃宏%鄔麗紅%陳一彊
량열%후장춘%황굉%오려홍%진일강
高迁移率族蛋白B1 (HMGB1)%人支气管上皮细胞(16HBE)%肿瘤坏死因子-α(TNF-α)%高级糖基化终产物受体(RAGE)
高遷移率族蛋白B1 (HMGB1)%人支氣管上皮細胞(16HBE)%腫瘤壞死因子-α(TNF-α)%高級糖基化終產物受體(RAGE)
고천이솔족단백B1 (HMGB1)%인지기관상피세포(16HBE)%종류배사인자-α(TNF-α)%고급당기화종산물수체(RAGE)
High mobility group box 1 (HMGB1)%human bronchial epithelial cells%tumor necrosis factorα(TNF-α)%receptor for advanced glycation end products(RAGE)
目的：探讨高迁移率族蛋白B1（HMGB1）对人支气管上皮细胞（16HBE）肿瘤坏死因子－α（TNF-α）表达的影响及相关机制。方法：（1）设置HMGB1不同浓度（0、100、500、2000 ng／mL）组；（2）设置高级糖基化终产物受体（RAGE）拮抗组：对照，HMGB12000 ng／mL，anti-RAGE，anti-RAGE＋HMGB1。用实时荧光定量PCR检测16HBETNF-αmRNA的表达，双抗体夹心酶联免疫吸附实验法（ELISA）检测细胞培养上清TNF-α的浓度，蛋白质印迹法（Western blotting）检测RAGE蛋白表达。结果：（1）16HBETNF-α的表达随HMGB1浓度的增大而增加；（2）16HBE的RAGE蛋白表达随HMGB1浓度的增大而增加；（3）相对于HMGB12000 ng／mL刺激组，anti-RAGE＋HMGB1组TNF-α表达明显减少。结论：HMGB1通过结合RAGE受体可以浓度依赖形式增加16HBETNF-α的表达。
目的：探討高遷移率族蛋白B1（HMGB1）對人支氣管上皮細胞（16HBE）腫瘤壞死因子－α（TNF-α）錶達的影響及相關機製。方法：（1）設置HMGB1不同濃度（0、100、500、2000 ng／mL）組；（2）設置高級糖基化終產物受體（RAGE）拮抗組：對照，HMGB12000 ng／mL，anti-RAGE，anti-RAGE＋HMGB1。用實時熒光定量PCR檢測16HBETNF-αmRNA的錶達，雙抗體夾心酶聯免疫吸附實驗法（ELISA）檢測細胞培養上清TNF-α的濃度，蛋白質印跡法（Western blotting）檢測RAGE蛋白錶達。結果：（1）16HBETNF-α的錶達隨HMGB1濃度的增大而增加；（2）16HBE的RAGE蛋白錶達隨HMGB1濃度的增大而增加；（3）相對于HMGB12000 ng／mL刺激組，anti-RAGE＋HMGB1組TNF-α錶達明顯減少。結論：HMGB1通過結閤RAGE受體可以濃度依賴形式增加16HBETNF-α的錶達。
목적：탐토고천이솔족단백B1（HMGB1）대인지기관상피세포（16HBE）종류배사인자－α（TNF-α）표체적영향급상관궤제。방법：（1）설치HMGB1불동농도（0、100、500、2000 ng／mL）조；（2）설치고급당기화종산물수체（RAGE）길항조：대조，HMGB12000 ng／mL，anti-RAGE，anti-RAGE＋HMGB1。용실시형광정량PCR검측16HBETNF-αmRNA적표체，쌍항체협심매련면역흡부실험법（ELISA）검측세포배양상청TNF-α적농도，단백질인적법（Western blotting）검측RAGE단백표체。결과：（1）16HBETNF-α적표체수HMGB1농도적증대이증가；（2）16HBE적RAGE단백표체수HMGB1농도적증대이증가；（3）상대우HMGB12000 ng／mL자격조，anti-RAGE＋HMGB1조TNF-α표체명현감소。결론：HMGB1통과결합RAGE수체가이농도의뢰형식증가16HBETNF-α적표체。
Objective To investigate the effect of high-mobility group box protein1 (HMGB1) on the expression of TNF-αand its mechanism in 16HBE in vitro. Methods groups with different HMGB1 (0, 100, 500, 2 000 ng/mL) concentration was set; RAGE antagonizing groups were as control, HMGB1-2000ng, anti-RAGE and anti-RAGE+HMGB1. The changes of TNF-αmRNA and secretion were determined by quantitative PCR and ELISA. RAGE protein level was measured by western blotting. Results HMGB1 intervention and TNF-α expression of 16HBE presented a positive dose-dependent relationship. Thechanges of RAGE was HMGB1positively concentration dependent. In comparison with HMGB1 2 000 ng/mL group, anti-RAGE+HMGB1showed a remarkable reduction of TNF-α secretion. Conclusion In vitro, HMGB1 increases TNF-α expression in 16HBE with a dose-dependent manner through RAGE.
