CAJ | 학술논문

以黄瓜CBF1基因沉默植株为试材，研究了冷胁迫下黄瓜基因的沉默对植株相关耐冷指标的影响。结果表明，与冷胁迫和常温条件下的正常植株相比，冷胁迫后的基因沉默植株的相对电导率和丙二醛含量均显著增加，叶绿素含量显著降低；冷胁迫后的基因沉默植株体内脯氨酸和可溶性蛋白含量增加的幅度远远低于冷胁迫后的正常植株增加的幅度。
이황과CBF1기인침묵식주위시재，연구료랭협박하황과기인적침묵대식주상관내랭지표적영향。결과표명，여랭협박화상온조건하적정상식주상비，랭협박후적기인침묵식주적상대전도솔화병이철함량균현저증가，협록소함량현저강저；랭협박후적기인침묵식주체내포안산화가용성단백함량증가적폭도원원저우랭협박후적정상식주증가적폭도。
Taking cucumber plants with CBF1 gene silenced as experiment material, we studied the effects of cucumber gene silencing on relative cold resistant indexes under cold stress. The results showed that, comparing with those under normal temperature, the relative conductivity and MDA content of cucumber with gene silencing were significantly higher, as well as chlorophyll content was significantly lower under cold stress. Moreover, under cold stress, the increasing amplitude of soluble protein and proline contents after gene silencing was lower than cucumber plants without gene silencing.