山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
44期
29-31
,共3页
肺肿瘤%微小RNA 100%微小RNA 148a%转移
肺腫瘤%微小RNA 100%微小RNA 148a%轉移
폐종류%미소RNA 100%미소RNA 148a%전이
lung cancer%miR-10%miR-148a%metastasis
目的：观察微小RNA 100（miR-100）、微小RNA 148a（miR-148a）在肺癌细胞与组织中的表达，并探讨其临床意义。方法采用RT-PCR方法检测人肺癌细胞系NCI-H1299、NCI-H358、H460、A549及人正常支气管上皮细胞系HBE，以及肺癌及其癌旁组织中miR-100、miR-148a表达；分析肺癌组织中miR-100、miR-148a表达与其临床病理参数的关系。结果与HBE细胞相比，miR-100在NCI-H1299、NCI-H358及A549细胞中表达升高，在H460细胞表达降低，P均＜0．05；miR-148a在NCI-H358、H460细胞中表达升高，在A549细胞中表达降低，P均＜0．05。与癌旁组织相比，肺癌组织中miR-100表达降低，但差异无统计学意义（P＝0．400）；miR-148a表达升高（P＝0．036）。肺癌组织中miR-100、miR-148a表达水平与患者性别、年龄、病理类型无关；有淋巴结转移者肺癌组织中miR-100表达水平较无淋巴结转移者低（P＝0．016）。结论 miR-100、miR-148a在肺癌细胞中表达异常；在肺癌组织中，miR-100表达降低，miR-148a表达增加，二者可能参与肺癌的发生、发展。
目的：觀察微小RNA 100（miR-100）、微小RNA 148a（miR-148a）在肺癌細胞與組織中的錶達，併探討其臨床意義。方法採用RT-PCR方法檢測人肺癌細胞繫NCI-H1299、NCI-H358、H460、A549及人正常支氣管上皮細胞繫HBE，以及肺癌及其癌徬組織中miR-100、miR-148a錶達；分析肺癌組織中miR-100、miR-148a錶達與其臨床病理參數的關繫。結果與HBE細胞相比，miR-100在NCI-H1299、NCI-H358及A549細胞中錶達升高，在H460細胞錶達降低，P均＜0．05；miR-148a在NCI-H358、H460細胞中錶達升高，在A549細胞中錶達降低，P均＜0．05。與癌徬組織相比，肺癌組織中miR-100錶達降低，但差異無統計學意義（P＝0．400）；miR-148a錶達升高（P＝0．036）。肺癌組織中miR-100、miR-148a錶達水平與患者性彆、年齡、病理類型無關；有淋巴結轉移者肺癌組織中miR-100錶達水平較無淋巴結轉移者低（P＝0．016）。結論 miR-100、miR-148a在肺癌細胞中錶達異常；在肺癌組織中，miR-100錶達降低，miR-148a錶達增加，二者可能參與肺癌的髮生、髮展。
목적：관찰미소RNA 100（miR-100）、미소RNA 148a（miR-148a）재폐암세포여조직중적표체，병탐토기림상의의。방법채용RT-PCR방법검측인폐암세포계NCI-H1299、NCI-H358、H460、A549급인정상지기관상피세포계HBE，이급폐암급기암방조직중miR-100、miR-148a표체；분석폐암조직중miR-100、miR-148a표체여기림상병리삼수적관계。결과여HBE세포상비，miR-100재NCI-H1299、NCI-H358급A549세포중표체승고，재H460세포표체강저，P균＜0．05；miR-148a재NCI-H358、H460세포중표체승고，재A549세포중표체강저，P균＜0．05。여암방조직상비，폐암조직중miR-100표체강저，단차이무통계학의의（P＝0．400）；miR-148a표체승고（P＝0．036）。폐암조직중miR-100、miR-148a표체수평여환자성별、년령、병리류형무관；유림파결전이자폐암조직중miR-100표체수평교무림파결전이자저（P＝0．016）。결론 miR-100、miR-148a재폐암세포중표체이상；재폐암조직중，miR-100표체강저，miR-148a표체증가，이자가능삼여폐암적발생、발전。
Objective To investigate the expression and clinical significance of miR-100 and miR-148a in lung cancer cells and tissues.Methods RT-PCR method was used to detect the expression of miR-100 and miR-148a in lung cancer cell lines NCI-H1299, NCI-H358, H460, A549 and normal human bronchial epithelial cell line HBE, and in the lung cancer tissues and adjacent tissues.To analyze the relationship between the expression of miR-100 and miR-148a and clini-copathological parameters of lung cancer.Results Compared with HBE cells, miR-100 expression in NCI-H1299, NCI-H358 and A549 increased significantly, decreased in H460, all P<0.05; the expression of miR-148a in NCI-H358 and H460 increased, decreased in A549, all P<0.05.Compared to non-tumor tissues, miR-100 expression was reduced in lung cancer, but the difference was not statistically significant (P=0.400);while the expression of miR-148a was signifi-cantly increased (P=0.036).miR-100 expression in lung cancer tissue with lymph node metastasis was lower than that in lung cancer tissue without lymph node metastasis (P=0.016).Conclusions The expression of miR-148a, miR-100 were aberrantly changed in lung cancer cells.miR-100 expression decreased in lung cancer tissues, miR-148a increased, which both maight be involved in the tumorigenesis of lung cancer.
