临床医学工程
臨床醫學工程
림상의학공정
CLINICAL MEDICAL ENGINEERING
2014年
5期
562-564
,共3页
王晓燕%田文洪%蔡艳霞%赵友恒%杨仁池
王曉燕%田文洪%蔡豔霞%趙友恆%楊仁池
왕효연%전문홍%채염하%조우항%양인지
瘦素%Mo7e细胞%增殖分化%凋亡
瘦素%Mo7e細胞%增殖分化%凋亡
수소%Mo7e세포%증식분화%조망
Leptin%Cell M07e%Proliferation%Apoptosis
目的:探讨瘦素对巨核细胞白血病的影响。方法以急性巨核细胞白血病细胞株M07藻为研究对象。运用砸栽-孕悦砸检测M07藻细胞瘦素受体皂砸晕A表达,运用月则凿怎-耘蕴陨杂A法检测瘦素对M07藻细胞的增殖作用,运用A灶灶藻曾蚤灶灾/孕陨双标流式检测瘦素对M07藻细胞的凋亡作用。结果瘦素受体长型韵遭-砸蕴和短型韵遭-砸杂在M07藻细胞均有皂砸晕A表达。瘦素对M07藻细胞的增殖有促进作用,并呈现一定的剂量依赖性,浓度为5灶早/皂L即显示出促增殖效应(孕越0.037),但浓度在5、10、25灶早/皂蕴之间无明显统计学差异,浓度为100灶早/皂蕴时其增殖效应最大。在时间依赖效应上,瘦素作用48澡,其细胞增殖明显高于作用24澡,而作用72澡其细胞增殖介于两者之间(孕越0.000)。瘦素对M07藻细胞具有抑制凋亡作用(孕越0.001),而且在作用72h后凋亡率最高。结论瘦素通过促进M07藻细胞的增殖,抑制其凋亡来参与巨核细胞白血病的发生、发展。
目的:探討瘦素對巨覈細胞白血病的影響。方法以急性巨覈細胞白血病細胞株M07藻為研究對象。運用砸栽-孕悅砸檢測M07藻細胞瘦素受體皂砸暈A錶達,運用月則鑿怎-耘蘊隕雜A法檢測瘦素對M07藻細胞的增殖作用,運用A竈竈藻曾蚤竈災/孕隕雙標流式檢測瘦素對M07藻細胞的凋亡作用。結果瘦素受體長型韻遭-砸蘊和短型韻遭-砸雜在M07藻細胞均有皂砸暈A錶達。瘦素對M07藻細胞的增殖有促進作用,併呈現一定的劑量依賴性,濃度為5竈早/皂L即顯示齣促增殖效應(孕越0.037),但濃度在5、10、25竈早/皂蘊之間無明顯統計學差異,濃度為100竈早/皂蘊時其增殖效應最大。在時間依賴效應上,瘦素作用48澡,其細胞增殖明顯高于作用24澡,而作用72澡其細胞增殖介于兩者之間(孕越0.000)。瘦素對M07藻細胞具有抑製凋亡作用(孕越0.001),而且在作用72h後凋亡率最高。結論瘦素通過促進M07藻細胞的增殖,抑製其凋亡來參與巨覈細胞白血病的髮生、髮展。
목적:탐토수소대거핵세포백혈병적영향。방법이급성거핵세포백혈병세포주M07조위연구대상。운용잡재-잉열잡검측M07조세포수소수체조잡훈A표체,운용월칙착즘-운온운잡A법검측수소대M07조세포적증식작용,운용A조조조증조조재/잉운쌍표류식검측수소대M07조세포적조망작용。결과수소수체장형운조-잡온화단형운조-잡잡재M07조세포균유조잡훈A표체。수소대M07조세포적증식유촉진작용,병정현일정적제량의뢰성,농도위5조조/조L즉현시출촉증식효응(잉월0.037),단농도재5、10、25조조/조온지간무명현통계학차이,농도위100조조/조온시기증식효응최대。재시간의뢰효응상,수소작용48조,기세포증식명현고우작용24조,이작용72조기세포증식개우량자지간(잉월0.000)。수소대M07조세포구유억제조망작용(잉월0.001),이차재작용72h후조망솔최고。결론수소통과촉진M07조세포적증식,억제기조망래삼여거핵세포백혈병적발생、발전。
Objective To explore whether leptin plays a role in megakaryocytic leukemia. Methods Megakaryocytic leukemia cell line M07e was used in this study. The mRNA expression of leptin receptor (Ob-R) in M07e was detected by RT-PCR. The proliferative effect of leptin on cell M07e was investigated with Brdu-ELISA and the apoptosis of cells was measured with flow cytometry after Annexin V labeling and PI staining. Results There was mRNA expression of Ob-RL and Ob-RS in M07e. Leptin promoted the proliferation of M07e in a dose and time dependent manner. The effect of proliferation was the most significant at the concentration of 100 ng/mL and after cultured for 48 hours. Leptin inhibited the apoptosis of M07e (P = 0.001), and the highest rate of apoptosis appeared after 72 hours. Conclusions Leptin may play an important role in human megakaryocytic leukemia by promoting the proliferation and inhibiting the apoptosis of M07e.