CAJ | 학술논문

目的：检测EB病毒相关性胃癌(耘月灾葬G悦)的阳性率及分析其临床病理特征，探讨耘月灾葬G悦微血管生成、微淋巴管生成的可能机制。方法利用-位分子杂交技术检测耘月灾编码小砸晕A1，从600例胃癌组织标本中，筛选出耘月病毒相关性胃癌组织标本，对其采用免疫组化方法检测蕴M孕1、月匀砸云1、灾耘G云-悦、蕴再灾耘-1和悦阅34的表达水平，分析其可能存在的关系。结果在600例胃癌标本中耘月灾葬G悦的阳性率为5.0豫，临床病理因素分析发现耘月灾葬G悦与性别、病理类型、发生部位及淋巴结转移有关(孕<0.05)，而与年龄无关(孕>0.05)。在30例耘月灾葬G悦标本中蕴M孕1呈低表达，与性别、分期及周围淋巴结转移无关(孕>0.05)，而月匀砸云1、灾耘G云-悦高表达，均与栽晕M分期和周围淋巴结转移有明显的关系(孕<0.05)；耘月灾葬G悦中M灾阅与性别及周围淋巴结转移无关，而与栽晕M分期显著有关(孕<0.05)；M蕴灾阅与栽晕M分期和周围淋巴结转移密切相关(孕<0.05)。月匀砸云1的表达与M灾阅无显著关系，而与M蕴灾阅有明显差异(孕<0.05)。灾耘G云-悦阳性表达的19例耘月灾葬G悦组织中，M灾阅和M蕴灾阅均明显高于阴性组，两组比较其差异具有显著的统计学意义(孕<0.05)。结论耘月灾葬G悦与性别、病理类型、发生部位及淋巴结转移有关，而与年龄无关。耘月灾葬G悦中蕴M孕1的表达率低，月匀砸云1的表达率高，可能与耘月灾葬G悦较少发生淋巴结转移有关。灾耘G云-悦可能参与耘月灾葬G悦的微血管和微淋巴管生成，其高表达间接促进肿瘤细胞沿新生的淋巴管迁徙和转移。
목적：검측EB병독상관성위암(운월재장G열)적양성솔급분석기림상병리특정，탐토운월재장G열미혈관생성、미림파관생성적가능궤제。방법이용-위분자잡교기술검측운월재편마소잡훈A1，종600례위암조직표본중，사선출운월병독상관성위암조직표본，대기채용면역조화방법검측온M잉1、월균잡운1、재운G운-열、온재재운-1화열열34적표체수평，분석기가능존재적관계。결과재600례위암표본중운월재장G열적양성솔위5.0예，림상병리인소분석발현운월재장G열여성별、병리류형、발생부위급림파결전이유관(잉<0.05)，이여년령무관(잉>0.05)。재30례운월재장G열표본중온M잉1정저표체，여성별、분기급주위림파결전이무관(잉>0.05)，이월균잡운1、재운G운-열고표체，균여재훈M분기화주위림파결전이유명현적관계(잉<0.05)；운월재장G열중M재열여성별급주위림파결전이무관，이여재훈M분기현저유관(잉<0.05)；M온재열여재훈M분기화주위림파결전이밀절상관(잉<0.05)。월균잡운1적표체여M재열무현저관계，이여M온재열유명현차이(잉<0.05)。재운G운-열양성표체적19례운월재장G열조직중，M재열화M온재열균명현고우음성조，량조비교기차이구유현저적통계학의의(잉<0.05)。결론운월재장G열여성별、병리류형、발생부위급림파결전이유관，이여년령무관。운월재장G열중온M잉1적표체솔저，월균잡운1적표체솔고，가능여운월재장G열교소발생림파결전이유관。재운G운-열가능삼여운월재장G열적미혈관화미림파관생성，기고표체간접촉진종류세포연신생적림파관천사화전이。
Objective To detect the positive rate of Epstein-Barr virus-associated gastric carcinoma (EBVaGC) in gastric carcinoma and analyze the clinical and pathological features, and explore the possible mechanisms with the process of angiogenesis and lymphangion-genesis in patients with EBVaGC. Methods We selected 30 cases of EBVaGC tissue from 600 cases of gastric cancer tissue by in situ hy-bridization of small RNA1 EBV-encoded, and detected the expression of LMP1, BHRF1, VEGF-C, LYVE-1 and CD34 in EBVaGC tissue specimens using immunohistochemical staining and then analyzed the correlations between them. Results The positive rate of EBVaGC in 600 cases of gastric cancer specimens was 5.0%, the analysis of clinical and pathological factors found EBVaGC had relations with gender, histological type, location and lymph node metastasis (P<0.05), but not related to age (P>0.05). The expression of LMP1 was low in 30 cas-es, and had no relations with gender, staging and lymph node metastasis. However, the expressions of BHRF1 and VEGF-C were high, and had obvious relations with TNM staging and lymph node metastasis (P<0.05). MVD in EBVaGC had no relations with gender and lymph node metastasis, but had obvious relations with TNM staging (P<0.05). MLVD was closely related to TNM staging and lymph node metas-tasis (P<0.05). The expression of BHRF1 was not related to MVD, but was closely related to MLVD (P<0.05). VEGF-C positive expression in 19 cases of EBVaGC tissue, expressions of MVD and MLVD were both higher than that of negative group, and there were statistical dif-ference between two groups (P<0.05). Conclusions EBVaGC has relations with gender, histological type, location and lymph node metas-tasis, but not with age. Low expression of LMP1 and high expression of BHRF1 in EBVaGC which may be related to less lymph node metastasis in EBVaGC occurred. Over-expression of VEGF-C demonstrates that VEGF-C may be involved in angiogenesis and lymphangio-genesis of EBVaGC, which indirectly promotes tumor cell's migra-tion and metastasis along the new lymphatic vessel.