CAJ | 학술논문

目的：探讨尾侧型同源转录因子2(caudal type homeobox transcription factor-2,CDX2)和黏蛋白2(mucin 2, MUC2)的表达与萎缩性胃炎和胃癌的关系。方法采用组织芯片技术构建含30例正常胃黏膜、30例萎缩性胃炎和41例胃癌的组织芯片,用免疫组化方法(S-P 法)检测组织芯片中 CDX2和 MUC2的表达。结果正常胃黏膜组织中CDX2、MUC2都无表达。萎缩性胃炎中 CDX2(100%)、MUC2(72.40%)高表达。胃癌黏膜 CDX2(66.67%)、MUC2(13.89%)阳性表达。CDX2、MUC2在不同胃黏膜阳性表达率差异有统计学意义(P <0.001)。CDX2和 MUC2在胃癌组织中的表达与病人年龄、浸润深度、有无淋巴结转移、TNM 分期均无关(P >0.05)。CDX2表达在男女性别差异有统计学意义(P <0.05)。CDX2表达与组织学类型、分化程度都无关(P >0.05)。MUC2表达在不同组织学类型、分化程度差异有统计学意义(P <0.05)。CDX2、MUC2二者的表达无明显相关性。结论 CDX2、MUC2表达在胃黏膜肠化生进展及胃癌发生中有重要作用。
목적：탐토미측형동원전록인자2(caudal type homeobox transcription factor-2,CDX2)화점단백2(mucin 2, MUC2)적표체여위축성위염화위암적관계。방법채용조직심편기술구건함30례정상위점막、30례위축성위염화41례위암적조직심편,용면역조화방법(S-P 법)검측조직심편중 CDX2화 MUC2적표체。결과정상위점막조직중CDX2、MUC2도무표체。위축성위염중 CDX2(100%)、MUC2(72.40%)고표체。위암점막 CDX2(66.67%)、MUC2(13.89%)양성표체。CDX2、MUC2재불동위점막양성표체솔차이유통계학의의(P <0.001)。CDX2화 MUC2재위암조직중적표체여병인년령、침윤심도、유무림파결전이、TNM 분기균무관(P >0.05)。CDX2표체재남녀성별차이유통계학의의(P <0.05)。CDX2표체여조직학류형、분화정도도무관(P >0.05)。MUC2표체재불동조직학류형、분화정도차이유통계학의의(P <0.05)。CDX2、MUC2이자적표체무명현상관성。결론 CDX2、MUC2표체재위점막장화생진전급위암발생중유중요작용。
Objective To investigate the expression of caudal type homeobox transcription factor - 2 (CDX2)and mucin 2 (MUC2)in atrophic gastritis and gastric carcinoma,and their correlation with atrophic gastritis and gastric carcinoma. Methods Tissue chips containing 30 atrophic gastritis specimens,41 gastric carcinoma specimens,and 30 normal gastric mucosa specimens were constructed by tissue microarray technolo-gy,at the same time,the expressions of CDX2 and MUC2 in tissue microarrays were determined by using im-munohistochemistry. Results Neither CDX2 expression nor MUC2 expression occurred in normal gastric mucosa tissue specimens.High expression of CDX2 (100%)and MUC2 (72.40%)occurred in atrophic gastri-tis specimens.Gastric carcinoma mucosa had CDX2 -positive expression (66.67%)and MUC2 -positive ex-pression (13.89%).The comparison of CDX2- and MUC2- positive expression among different gastric mu-cosa specimens yielded statistical significant differences (P <0.001 ).The expression of CDX2 and MUC2 in gastric carcinoma tissues had no association with patients'age,depth of invasion,lymph nodes metastasis and TNM staging (P >0.05).Compared the males with CDX2 -positive gastric cancer to that of females,there was statistically significant difference (P <0.05).The expression of CDX2 had no association with histological type,tumor differentiation grade (P >0.05).The expression of MUC2 in tumors with different histological type,different differentiation yielded statistically significant difference (P <0.05).The expression of CDX2 had no positively correlation with MUC2. Conclusion The expression of CDX2 and MUC2 plays an impor-tant role in the development of gastric mucosa intestinal metaplasia,also in the development of gastric carcino-ma.