右江民族医学院学报
右江民族醫學院學報
우강민족의학원학보
JOURNAL OF YOUJIANG MEDICAL COLLEGE FOR NATIONALITIES
2014年
5期
679-681
,共3页
韦红玉%隆昕颖%凌俊%赵丽娟%谢振锋%唐华英%韦连登%陈源红%覃艳春
韋紅玉%隆昕穎%凌俊%趙麗娟%謝振鋒%唐華英%韋連登%陳源紅%覃豔春
위홍옥%륭흔영%릉준%조려연%사진봉%당화영%위련등%진원홍%담염춘
结核分枝杆菌%利福平%利福平耐药决定区%rpoB基因
結覈分枝桿菌%利福平%利福平耐藥決定區%rpoB基因
결핵분지간균%리복평%리복평내약결정구%rpoB기인
Mycobacterium tuberculosis%rifampin%rifampin resistance determining region%rpoB gene
目的:分析百色地区结核分枝杆菌(MTB)利福平耐药相关基因 rpoB的突变特征。方法收集128例结核病患者痰液标本,采用改良罗氏培养基分离培养结核分枝杆菌,绝对浓度法检测利福平耐药性,PCR 技术检测耐药结核分枝杆菌 rpoB基因序列并测序分析利福平耐药相关基因 rpoB的突变特征。结果分离培养获得98例 MTB,98例 MTB出现36株利福平耐药,耐药率为36.73%,36株耐利福平菌株中有27株 rpoB基因发生突变,基因突变率为75.00%,其中516位点突变率为3.70%(1/27),531位点突变率为59.26%(16/27),526位点突变率为29.63%(8/27),533位点突变率为7.41%(2/27)。突变热点依次为 rpoB531、rpoB526和 rpoB533。结论百色地区耐利福平结核分枝杆菌耐药性产生的主要分子机制是因为 rpoB的基因发生突变。
目的:分析百色地區結覈分枝桿菌(MTB)利福平耐藥相關基因 rpoB的突變特徵。方法收集128例結覈病患者痰液標本,採用改良囉氏培養基分離培養結覈分枝桿菌,絕對濃度法檢測利福平耐藥性,PCR 技術檢測耐藥結覈分枝桿菌 rpoB基因序列併測序分析利福平耐藥相關基因 rpoB的突變特徵。結果分離培養穫得98例 MTB,98例 MTB齣現36株利福平耐藥,耐藥率為36.73%,36株耐利福平菌株中有27株 rpoB基因髮生突變,基因突變率為75.00%,其中516位點突變率為3.70%(1/27),531位點突變率為59.26%(16/27),526位點突變率為29.63%(8/27),533位點突變率為7.41%(2/27)。突變熱點依次為 rpoB531、rpoB526和 rpoB533。結論百色地區耐利福平結覈分枝桿菌耐藥性產生的主要分子機製是因為 rpoB的基因髮生突變。
목적:분석백색지구결핵분지간균(MTB)리복평내약상관기인 rpoB적돌변특정。방법수집128례결핵병환자담액표본,채용개량라씨배양기분리배양결핵분지간균,절대농도법검측리복평내약성,PCR 기술검측내약결핵분지간균 rpoB기인서렬병측서분석리복평내약상관기인 rpoB적돌변특정。결과분리배양획득98례 MTB,98례 MTB출현36주리복평내약,내약솔위36.73%,36주내리복평균주중유27주 rpoB기인발생돌변,기인돌변솔위75.00%,기중516위점돌변솔위3.70%(1/27),531위점돌변솔위59.26%(16/27),526위점돌변솔위29.63%(8/27),533위점돌변솔위7.41%(2/27)。돌변열점의차위 rpoB531、rpoB526화 rpoB533。결론백색지구내리복평결핵분지간균내약성산생적주요분자궤제시인위 rpoB적기인발생돌변。
Objective To analyze rpoB gene mutation characteristics in rifampin-resistant related Myco-bacterium tuberculosis (MTB)in Baise city. Methods The sputum specimens were collected from 128 pa-tients with tuberculosis and then were cultured on modifieded Lowenstein-Jensen culture medium,the drug sensitivity of rifampin was detected by absolute concentration method,mutation patterns of rpoB genes were detected by PCR and sequencing. Results Ninety-eight MTB isolates were obtained from 128 MTB clinical strains,36.73% (36/98)isolates were resistant to rifampin,27 of 36 rifampin-resistance MTB (75.00%)i-solates carried mutations in the rpoB gene,and 3.70% (1/27)isolates carried mutations in 516 site,59.26%(16/27)isolates carried mutations in 531 site,7.41% (2/27)isolates carried mutations in 533 site.The top 3 loci with high mutation frequency were rpoB531,rpoB526 and rpoB533. Conclusion The main mechanism of rifampin-resistant Mycobacterium tuberculos occurrence is caused by rpoB gene mutation in Mycobacterium tuberculosis in Baise city.