CAJ | 학술논문

以香血糯(紫色果皮)、02428(白色果皮)及以它们为亲本的衍生世代 F1、F2和 F3为材料,研究紫色果皮的遗传特征；根据已克隆的紫色果皮 Pb 与白色果皮 pb 等位基因第7外显子的差异,设计酶切扩增片段长度多态性标记(CAPS),分析标记基因型；选取10份材料 PCR 产物进行测序分析。结果表明,以白色果皮02428为母本时,F1的果皮颜色为白色,而以紫色果皮香血糯为母本时,杂交种 F1的果皮颜色为紫色；正反交 F1植株所结 F2种子果皮都为紫色,没有分离；F2植株所结F3种子果皮颜色发生分离,符合3∶1分离比例；CAPS 标记分析结果表明紫色果皮基因能被切开,而紫色颖壳基因不能切开,测序结果说明紫色果皮材料相对于白色果皮材料在第7外显子存在 GT 缺失。因此,水稻紫色果皮是单基因控制的显性遗传,由母体基因型决定,是典型的延迟遗传；紫色果皮与紫色颖壳由不同基因控制；紫色果皮基因的 CAPS 分子标记可以作为紫米育种的基因功能标记。
이향혈나(자색과피)、02428(백색과피)급이타문위친본적연생세대 F1、F2화 F3위재료,연구자색과피적유전특정；근거이극륭적자색과피 Pb 여백색과피 pb 등위기인제7외현자적차이,설계매절확증편단장도다태성표기(CAPS),분석표기기인형；선취10빈재료 PCR 산물진행측서분석。결과표명,이백색과피02428위모본시,F1적과피안색위백색,이이자색과피향혈나위모본시,잡교충 F1적과피안색위자색；정반교 F1식주소결 F2충자과피도위자색,몰유분리；F2식주소결F3충자과피안색발생분리,부합3∶1분리비례；CAPS 표기분석결과표명자색과피기인능피절개,이자색영각기인불능절개,측서결과설명자색과피재료상대우백색과피재료재제7외현자존재 GT 결실。인차,수도자색과피시단기인공제적현성유전,유모체기인형결정,시전형적연지유전；자색과피여자색영각유불동기인공제；자색과피기인적 CAPS 분자표기가이작위자미육충적기인공능표기。
In order to determine the heredity of purple pericarp,the purple pericarp cultivar Xiangxuenuo,developed in Jiangsu Province,and white pericarp cultivar 02428 were used as parents to make reciprocal crosses,and the colour of the seeds of relevant F1 ,F2 ,F3 generation were phenotyped.The results indicated that the purple pericarp of Xiangxuenuo was controlled by single dominant nuclear gene,belonging to the typical maternal influence.Purple pericarp in rice was due to a 2 bp deletion in Pb gene on chromosome 4 which encoded a basic helix-loop-helix protein. A CAPS marker was developed based on the 2 bp deletion,and the CAPS marker was co-segregated with the colour of pericarp in the F2 population,allowing to identify the homozygous purple pericarp genotype,homozygous white pericarp genotype and heterozygous purple pericarp genotype individuals.The PCR products of 10 rice lines were sequenced.All the purple pericarp rice lines carried GT deletion.It was interesting that the wild rice lines with red pericarp carried the normal allele;therefore we speculated that the purple pericarp was mutated from cultivated rice. Furthermore,one line with purple hull and white pericarp carried pb allele,it showed the complex genetic mechanism in rice pigmentation.In conclusion,the CAPS marker is a perfect marker for purple pericarp rice breeding and the evolution of Pb gene need further research.