中国水稻科学
中國水稻科學
중국수도과학
CHINESE JOURNAL OF RICE SCIENCE
2014年
6期
559-569
,共11页
韩雪颖%杨勇%余初浪%张文浩%叶胜海%陈斌%程晨%程晔%严成其%陈剑平
韓雪穎%楊勇%餘初浪%張文浩%葉勝海%陳斌%程晨%程曄%嚴成其%陳劍平
한설영%양용%여초랑%장문호%협성해%진빈%정신%정엽%엄성기%진검평
水稻%类病变突变体%细胞程序性死亡%抗病性%荧光双向差异凝胶电脉
水稻%類病變突變體%細胞程序性死亡%抗病性%熒光雙嚮差異凝膠電脈
수도%류병변돌변체%세포정서성사망%항병성%형광쌍향차이응효전맥
rice%lesion-mimic mutant%programmed cell death%disease resistance%2D-DIGE
水稻类病变突变体 chl1具有抗病性增强的类病变表型,对水稻白叶枯病和稻瘟病都具有很强的抗性。利用蛋白质组学技术分析 chl1与其野生型之间的差异表达蛋白,探讨 chl1类病变表型的形成和抗病反应的分子机制。利用荧光双向差异凝胶电泳(two-dimensional fluorescence difference gel electrophoresis,2D-DIGE)技术和质谱分析,chl1中共鉴定到70个差异表达的蛋白点,包括46个上调蛋白点和24个下调蛋白点。这些蛋白点参与不同的生物过程,包括防御相关、光合作用、氧化还原、氨基酸/蛋白质代谢、分子伴侣、碳水化合物代谢。对这些差异表达蛋白进行生物信息分析,推测它们所在的复杂调控网络可能参与 chl1叶片细胞程序性死亡(programmed cell death,PCD)及其抗病性的调控。
水稻類病變突變體 chl1具有抗病性增彊的類病變錶型,對水稻白葉枯病和稻瘟病都具有很彊的抗性。利用蛋白質組學技術分析 chl1與其野生型之間的差異錶達蛋白,探討 chl1類病變錶型的形成和抗病反應的分子機製。利用熒光雙嚮差異凝膠電泳(two-dimensional fluorescence difference gel electrophoresis,2D-DIGE)技術和質譜分析,chl1中共鑒定到70箇差異錶達的蛋白點,包括46箇上調蛋白點和24箇下調蛋白點。這些蛋白點參與不同的生物過程,包括防禦相關、光閤作用、氧化還原、氨基痠/蛋白質代謝、分子伴侶、碳水化閤物代謝。對這些差異錶達蛋白進行生物信息分析,推測它們所在的複雜調控網絡可能參與 chl1葉片細胞程序性死亡(programmed cell death,PCD)及其抗病性的調控。
수도류병변돌변체 chl1구유항병성증강적류병변표형,대수도백협고병화도온병도구유흔강적항성。이용단백질조학기술분석 chl1여기야생형지간적차이표체단백,탐토 chl1류병변표형적형성화항병반응적분자궤제。이용형광쌍향차이응효전영(two-dimensional fluorescence difference gel electrophoresis,2D-DIGE)기술화질보분석,chl1중공감정도70개차이표체적단백점,포괄46개상조단백점화24개하조단백점。저사단백점삼여불동적생물과정,포괄방어상관、광합작용、양화환원、안기산/단백질대사、분자반려、탄수화합물대사。대저사차이표체단백진행생물신식분석,추측타문소재적복잡조공망락가능삼여 chl1협편세포정서성사망(programmed cell death,PCD)급기항병성적조공。
A lesion-mimic mutant in rice (Oryza sativa L.),chloroplastic-H 2 O 2-induced lesion 1 (chl1 ),has enhanced resistance to rice blast and bacterial blight.To understand the molecular mechanisms underlying this phenotype and its resistance responses,a proteomics-based approach was used to identify differentially-expressed proteins between chl1 and its wild type.Using two-dimensional fluorescence difference gel electrophoresis technology and mass spectrometry, 70 protein spots were successfully identified,of which 46 were up-regulated and 24 were down-regulated in the mutant. These differentially-expressed proteins are involved in diverse biological processes including disease resistance,photo-synthesis,oxidation-reduction reaction,amino acid/protein metabolism,chaperoning and carbohydrate metabolism. The complex regulatory network in which these proteins are involved may play an important role in regulating the pro-grammed cell death and the resistance reaction in chl1 .
