CAJ | 학술논문

目的：研究 Six1基因对人肺癌细胞 A549增殖和迁移能力的影响及其作用机制。方法将 Six1的特异小干扰 RNA序列转染入A549细胞中,应用Western印迹法检测Six1在A549细胞中的蛋白水平的表达情况,应用MTT法和细胞计数观察低表达 Six1对A549细胞增殖的作用,应用划痕实验和transwell侵袭小室实验检测A549细胞迁移能力的变化情况,应用 Western印迹法检测PI3K信号通路蛋白的表达变化。结果在 A549细胞中,特异的小干扰 RNA 使 Six1在蛋白水平表达降低,低表达 Six1的 A549细胞系增殖和迁移能力下降,p-AKT 的蛋白表达降低。结论在 A549细胞系中 Six1通过PI3K通路抑制 A549细胞的侵袭和转移能力。
목적：연구 Six1기인대인폐암세포 A549증식화천이능력적영향급기작용궤제。방법장 Six1적특이소간우 RNA서렬전염입A549세포중,응용Western인적법검측Six1재A549세포중적단백수평적표체정황,응용MTT법화세포계수관찰저표체 Six1대A549세포증식적작용,응용화흔실험화transwell침습소실실험검측A549세포천이능력적변화정황,응용 Western인적법검측PI3K신호통로단백적표체변화。결과재 A549세포중,특이적소간우 RNA 사 Six1재단백수평표체강저,저표체 Six1적 A549세포계증식화천이능력하강,p-AKT 적단백표체강저。결론재 A549세포계중 Six1통과PI3K통로억제 A549세포적침습화전이능력。
Objective To investigate the influence of Six1 on cell proliferation and migration in A549 cell line and its mechanism.Methods Six1 specific Si-RNA was transfected into human lung carcinoma cell line A549.We detected the efficien-cy of transfection by Western blot.The effects of si-Six1 on A549 cell growth was by means of MTT and cell count assay;The effects of Six1 on A549 cell migration was by means of cell scratch test and transwell assay;The protein expression of PI3K Sig-nal pathway was detected by western blot.Results In A549 cell line,the expression of Six1 was knocked-down by specific si-RNA,and down-regulation of Six1 decreased the expression of p-AKT,which led to decrease of the ability of proliferation and migration of A549.Conclusion Six1 inhibits the proliferation and migration of A549 cells through PI3K pathway.