分子诊断与治疗杂志
分子診斷與治療雜誌
분자진단여치료잡지
JOURNAL OF MOLECULAR DIAGNOSIS AND THERAPY
2014年
6期
410-414
,共5页
翁玉英倪琛%林秀凤%郑苗%黄海龙%HUANG hailong
翁玉英倪琛%林秀鳳%鄭苗%黃海龍%HUANG hailong
옹옥영예침%림수봉%정묘%황해룡%HUANG hailong
人巨细胞病毒%实时荧光定量PCR%婴儿%新生儿
人巨細胞病毒%實時熒光定量PCR%嬰兒%新生兒
인거세포병독%실시형광정량PCR%영인%신생인
Human cytomegalovirus%Fluorescent quantitative PCR%Infant%Newborn
目的:探讨尿液、淋巴细胞和母乳的 HCMV-DNA 检测以及血清 HCMV-IgM 检测在小儿人巨细胞病毒(HCMV)感染诊断中的意义。方法用实时荧光定量 PCR检测189例疑似HCMV感染患儿尿液、淋巴细胞和母乳的HCMV-DNA ,用化学发光法检测其血清HCMV-IgM ,并分年龄分性别对其阳性率进行分析比较。结果新生儿组母乳HCMV-DNA阳性检出率(60.3%)显著高于尿液(5.2%, P<0.001)和淋巴细胞(12.1%,P<0.001),也显著高于血清 HCMV-IgM 阳性率(3.4%,P<0.001);婴儿组母乳(48.1%)与尿液(55.0%)HCMV-DNA 阳性率差异无统计学意义(P>0.05),但两者均显著高于淋巴细胞 HCMV-DNA 阳性率(22.1%, P<0.001)和血清 HCMV-IgM 阳性率(24.4%, P<0.001);婴儿组的尿液 HCMV-DNA、血清 HCMV-IgM 阳性率均显著高于新生儿组( P<0.01);两组各指标男女阳性率差异均无统计学意义( P>0.05);母乳 HCMV-DNA 阳性率与尿液、淋巴细胞 HCMV-DNA 阳性率及血清 HCMV-IgM 阳性率均呈显著正相关(r =0.630,P =0.000;r =0.413,P =0.000;r =0.341,P =0.000)。结论联合尿液、淋巴细胞HCMV-DNA 和血清HCMV-IgM 检测有助于患儿HCMV 感染的辅助诊断;在无法联合检测的条件下,婴儿首选尿液 HCMV-DNA 检测可获得较高检出率;母乳 HCMV-DNA检测并正确喂养有助于预防HCMV感染。
目的:探討尿液、淋巴細胞和母乳的 HCMV-DNA 檢測以及血清 HCMV-IgM 檢測在小兒人巨細胞病毒(HCMV)感染診斷中的意義。方法用實時熒光定量 PCR檢測189例疑似HCMV感染患兒尿液、淋巴細胞和母乳的HCMV-DNA ,用化學髮光法檢測其血清HCMV-IgM ,併分年齡分性彆對其暘性率進行分析比較。結果新生兒組母乳HCMV-DNA暘性檢齣率(60.3%)顯著高于尿液(5.2%, P<0.001)和淋巴細胞(12.1%,P<0.001),也顯著高于血清 HCMV-IgM 暘性率(3.4%,P<0.001);嬰兒組母乳(48.1%)與尿液(55.0%)HCMV-DNA 暘性率差異無統計學意義(P>0.05),但兩者均顯著高于淋巴細胞 HCMV-DNA 暘性率(22.1%, P<0.001)和血清 HCMV-IgM 暘性率(24.4%, P<0.001);嬰兒組的尿液 HCMV-DNA、血清 HCMV-IgM 暘性率均顯著高于新生兒組( P<0.01);兩組各指標男女暘性率差異均無統計學意義( P>0.05);母乳 HCMV-DNA 暘性率與尿液、淋巴細胞 HCMV-DNA 暘性率及血清 HCMV-IgM 暘性率均呈顯著正相關(r =0.630,P =0.000;r =0.413,P =0.000;r =0.341,P =0.000)。結論聯閤尿液、淋巴細胞HCMV-DNA 和血清HCMV-IgM 檢測有助于患兒HCMV 感染的輔助診斷;在無法聯閤檢測的條件下,嬰兒首選尿液 HCMV-DNA 檢測可穫得較高檢齣率;母乳 HCMV-DNA檢測併正確餵養有助于預防HCMV感染。
목적:탐토뇨액、림파세포화모유적 HCMV-DNA 검측이급혈청 HCMV-IgM 검측재소인인거세포병독(HCMV)감염진단중적의의。방법용실시형광정량 PCR검측189례의사HCMV감염환인뇨액、림파세포화모유적HCMV-DNA ,용화학발광법검측기혈청HCMV-IgM ,병분년령분성별대기양성솔진행분석비교。결과신생인조모유HCMV-DNA양성검출솔(60.3%)현저고우뇨액(5.2%, P<0.001)화림파세포(12.1%,P<0.001),야현저고우혈청 HCMV-IgM 양성솔(3.4%,P<0.001);영인조모유(48.1%)여뇨액(55.0%)HCMV-DNA 양성솔차이무통계학의의(P>0.05),단량자균현저고우림파세포 HCMV-DNA 양성솔(22.1%, P<0.001)화혈청 HCMV-IgM 양성솔(24.4%, P<0.001);영인조적뇨액 HCMV-DNA、혈청 HCMV-IgM 양성솔균현저고우신생인조( P<0.01);량조각지표남녀양성솔차이균무통계학의의( P>0.05);모유 HCMV-DNA 양성솔여뇨액、림파세포 HCMV-DNA 양성솔급혈청 HCMV-IgM 양성솔균정현저정상관(r =0.630,P =0.000;r =0.413,P =0.000;r =0.341,P =0.000)。결론연합뇨액、림파세포HCMV-DNA 화혈청HCMV-IgM 검측유조우환인HCMV 감염적보조진단;재무법연합검측적조건하,영인수선뇨액 HCMV-DNA 검측가획득교고검출솔;모유 HCMV-DNA검측병정학위양유조우예방HCMV감염。
Objective To study the diagnostic significance of detection of human cytomegalovirus (HCMV) IgM in sera, and HCMV DNA in urine, blood lymphocytes and mother's breast milk for baby infection. Methods Chemiluminescence technology was used to detect the specific HCMV IgM antibody in sera and fluorescent quantitative PCR was used to detect HCMV DNA simultaneously in urine, blood lymphocytes and the mother's breast milk of 189 infants with suspected HCMV infection. The positive rates in these specimens were analyzed in either newborn group (less than 28 days old) or infant group (from 29 days to 12 months old), as well as boy group and girl group. Results In newborn group, the positive rate of HCMV DNA in mother's breast milk (60.3%) was higher than those both in urine (5.2%,P < 0.001) and in blood lymphocytes (12.1%, P < 0.001). In infant group, no statistical difference was detected between the positive rates of HCMV DNA in mother's breast milk (48.1%) and that in urine (55.0%), which were higher than that in blood lymphocytes (22.1%) and the positive rate of HCMV IgM in sera (24.4%). In infant group, the positive rate of HCMV DNA in urines and that of HCMV IgM in sera were higher than those in newborn group (P < 0.01). No statistical difference between boy group and girl group was detected in all specimens. The positive rate of HCMV DNA in mother's breast milk showed significant positive correlations with those of all other specimens (P < 0.001). Conclusions Combined detection of HCMV IgM in sera and HCMV DNA in urine, blood lymphocytes and mother's breast milk would contribute to auxiliary diagnosis of baby with HCMV infection. If it is not available to detect all these samples, detection of HCMV DNA in urine of infant may be the first choice. Detection of HCMV DNA in mother's breast milk and breastfeeding in a correct way would contribute to the prevention of HCMV infection.