CAJ | 학술논문

目的：探讨广藿香油对家兔内毒素性发热的解热作用及机制。方法：根据实验当天体重及基础体温将42雄性只家兔随机分为7组，分别为空白对照组、模型对照组、西药阳性组、中药阳性组及广藿香油高、中、低（2％、1％和0．5％）3个剂量组。除空白对照组外，其余6组均按1 mL/kg经耳缘静脉注射大肠杆菌内毒素（2 mg/L）复制家兔内毒素性发热模型，0．5 h后经耳缘静脉给药，空白和模型对照组注射等量的0．5％吐温80，西药、中药阳性组和广藿香油高、中、低剂量组分别注射对应剂量药物，观察各组家兔体温变化，采用放射免疫法测定血清中白细胞介素-1β（ IL-1β）和肿瘤坏死因子α（ TNF-α）以及下丘脑环磷酸腺苷（ cAMP）和前列腺素E2（ PGE2）的含量。结果：与空白对照组相比，模型对照组家兔体温、IL-1β、TNF-α、cAMP和PGE2显著上升（ P＜0．01或P＜0.05）；广藿香油对发热家兔的体温升高有明显的抑制作用，广藿香油高剂量组给药后1.5～5.5 h，家兔体温分别升高（1.06±1.55）、（1.62±1.36）、（1.38±1.22）、（0.98±0.98）和（0.48±0.95）℃，广藿香油中剂量组给药后3.5～5.5 h，体温分别升高（1.47±0.73）、（1.15±0.68）和（0.63±0.54）℃，广藿香油低剂量组给药后各时点均有降低发热家兔体温的趋势；广藿香油可明显抑制血清中TNF-α和下丘脑中cAMP含量的升高，有降低血清中IL-1β和下丘脑中PGE2含量升高的趋势。结论：广藿香油对家兔内毒性发热有显著解热作用，其机制可能与抑制血清中TNF-α和下丘脑中cAMP含量升高有关。
목적：탐토엄곽향유대가토내독소성발열적해열작용급궤제。방법：근거실험당천체중급기출체온장42웅성지가토수궤분위7조，분별위공백대조조、모형대조조、서약양성조、중약양성조급엄곽향유고、중、저（2％、1％화0．5％）3개제량조。제공백대조조외，기여6조균안1 mL/kg경이연정맥주사대장간균내독소（2 mg/L）복제가토내독소성발열모형，0．5 h후경이연정맥급약，공백화모형대조조주사등량적0．5％토온80，서약、중약양성조화엄곽향유고、중、저제량조분별주사대응제량약물，관찰각조가토체온변화，채용방사면역법측정혈청중백세포개소-1β（ IL-1β）화종류배사인자α（ TNF-α）이급하구뇌배린산선감（ cAMP）화전렬선소E2（ PGE2）적함량。결과：여공백대조조상비，모형대조조가토체온、IL-1β、TNF-α、cAMP화PGE2현저상승（ P＜0．01혹P＜0.05）；엄곽향유대발열가토적체온승고유명현적억제작용，엄곽향유고제량조급약후1.5～5.5 h，가토체온분별승고（1.06±1.55）、（1.62±1.36）、（1.38±1.22）、（0.98±0.98）화（0.48±0.95）℃，엄곽향유중제량조급약후3.5～5.5 h，체온분별승고（1.47±0.73）、（1.15±0.68）화（0.63±0.54）℃，엄곽향유저제량조급약후각시점균유강저발열가토체온적추세；엄곽향유가명현억제혈청중TNF-α화하구뇌중cAMP함량적승고，유강저혈청중IL-1β화하구뇌중PGE2함량승고적추세。결론：엄곽향유대가토내독성발열유현저해열작용，기궤제가능여억제혈청중TNF-α화하구뇌중cAMP함량승고유관。
[ ABSTRACT] AIM:To investigate the antipyretic effect of patchouli oil on lipopolysaccharide ( LPS)-induced fe-ver in rabbits.METHODS:Male rabbits (n=42) were randomly divided into 7 groups according to their body weight and basal body temperature, including control group, model group, western medical positive group, traditional Chinese medical positive group, and high, middle and low doses (2%, 1%and 0.5%) of patchouli oil groups.Subsequently, except the controls, the rabbits were injected with LPS at a dose of 1 mL/kg (2 mg/L) through marginal ear vein to establish rabbit fever model and the rabbits in control group received the same volume of NS.The rabbits in control group and model group were injected with 0.5%Tween-80 0.5 h late, and the rabbits in the other groups were treated with correspoonding drugs. The effect of patchouli oil on the body temperature was observed, and the levels of interleukin-1β( IL-1β) and tumor nec-rosis factor-α(TNF-α) in the serum, and prostaglandin E2(PGE2) and cyclic adenosine monophosphate (cAMP) in the hypothalamus were measured by radioimmunoassay.RESULTS: The body temperature and the levels of IL-1β, TNF-α, cAMP and PGE2 in model group were significant higher than those in control group.Patchouli oil notably inhibited the body temperature in the febrile rabbits.From 1.5 h to 5.5 h after administration, the body temperatures were increased by (1.06 ±1.55), (1.62 ±1.36), (1.38 ±1.22), (0.98 ±0.98) and (0.48 ±0.95) ℃in high patchouli oil group, re-spectively.From 3.5 to 5.5 h after administration, the body temperatures were elevated by ( 1.47 ±0.73 ) , ( 1.15 ± 0.68) and (0.63 ±0.54) ℃ in middle patchouli oil group, respectively.A tendency of downregulation of the elevated body temperatures was observed at every time point after administration in low patchouli oil group.Patchouli oil significantly decreased the levels of TNF-αin the serum and cAMP content in the hypothalamus, and attenuated the elevated tendency of the IL-1βlevel in the serum and PGE2 level in the hypothalamus.CONCLUSION:Patchouli oil evidently has antipyretic effect on LPS-induced fever in the rabbits.The antipyretic mechanism might be related to the inhibition of TNF-αlevel in serum and cAMP content in the hypothalamus.