中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
10期
1868-1872
,共5页
MicroRNA-16%类风湿关节炎%滑膜成纤维细胞%细胞因子类
MicroRNA-16%類風濕關節炎%滑膜成纖維細胞%細胞因子類
MicroRNA-16%류풍습관절염%활막성섬유세포%세포인자류
MicroRNA-16%Rheumatoid arthritis%Synovial fibroblasts%Cytokines
目的：研究microRNA-16（miR-16）对类风湿关节炎（rheumatoid arthritis，RA）患者滑膜成纤维细胞（ rheumatoid arthritis synovial fibroblasts，RASFs）增殖、侵袭及细胞因子分泌的影响。方法：体外分离培养RASFs，脂质体转染化学合成的miR-16 mimic或miR-16抑制剂，分别采用MTT法、Transwell小室法和流式细胞术检测其对RASFs增殖、侵袭及凋亡的影响；RT-PCR和Western blotting检测miR-16对RASFs 基质金属蛋白酶3/13（ matrix metalloproteinase 3/13，MMP3/13）及白细胞介素1β（ interleukin 1β，IL-1β）表达的影响。结果：增殖实验结果表明miR-16可显著抑制RASFs的增殖；细胞侵袭结果表明miR-16可显著抑制RASFs的侵袭；流式细胞术检测发现miR-16对RASFs凋亡无显著影响；miR-16可下调MMP3/13及IL-1β的表达水平。结论：miR-16在RA的发生中起着重要作用，它可能通过下调MMP3/13及IL-1β的表达抑制RASFs增殖和侵袭。这为进一步研究miR-16在RA中的作用机制奠定了基础。
目的：研究microRNA-16（miR-16）對類風濕關節炎（rheumatoid arthritis，RA）患者滑膜成纖維細胞（ rheumatoid arthritis synovial fibroblasts，RASFs）增殖、侵襲及細胞因子分泌的影響。方法：體外分離培養RASFs，脂質體轉染化學閤成的miR-16 mimic或miR-16抑製劑，分彆採用MTT法、Transwell小室法和流式細胞術檢測其對RASFs增殖、侵襲及凋亡的影響；RT-PCR和Western blotting檢測miR-16對RASFs 基質金屬蛋白酶3/13（ matrix metalloproteinase 3/13，MMP3/13）及白細胞介素1β（ interleukin 1β，IL-1β）錶達的影響。結果：增殖實驗結果錶明miR-16可顯著抑製RASFs的增殖；細胞侵襲結果錶明miR-16可顯著抑製RASFs的侵襲；流式細胞術檢測髮現miR-16對RASFs凋亡無顯著影響；miR-16可下調MMP3/13及IL-1β的錶達水平。結論：miR-16在RA的髮生中起著重要作用，它可能通過下調MMP3/13及IL-1β的錶達抑製RASFs增殖和侵襲。這為進一步研究miR-16在RA中的作用機製奠定瞭基礎。
목적：연구microRNA-16（miR-16）대류풍습관절염（rheumatoid arthritis，RA）환자활막성섬유세포（ rheumatoid arthritis synovial fibroblasts，RASFs）증식、침습급세포인자분비적영향。방법：체외분리배양RASFs，지질체전염화학합성적miR-16 mimic혹miR-16억제제，분별채용MTT법、Transwell소실법화류식세포술검측기대RASFs증식、침습급조망적영향；RT-PCR화Western blotting검측miR-16대RASFs 기질금속단백매3/13（ matrix metalloproteinase 3/13，MMP3/13）급백세포개소1β（ interleukin 1β，IL-1β）표체적영향。결과：증식실험결과표명miR-16가현저억제RASFs적증식；세포침습결과표명miR-16가현저억제RASFs적침습；류식세포술검측발현miR-16대RASFs조망무현저영향；miR-16가하조MMP3/13급IL-1β적표체수평。결론：miR-16재RA적발생중기착중요작용，타가능통과하조MMP3/13급IL-1β적표체억제RASFs증식화침습。저위진일보연구miR-16재RA중적작용궤제전정료기출。
AIM:To investigate the effect of microRNA-16 ( miR-16) on the proliferation, invasion and cyto-kine secretion of rheumatoid arthritis ( RA) synovial fibroblasts ( RASFs) from the RA patients.METHODS: miR-16 mimic and miR-16 inhibitor were synthesized, and then Transfected into RASFs isolated from RA patients with lipo-fectamine.MTT assay, Transwell chamber and flow cytometry were used to determine the effect of miR-16 on proliferation, invasion and apoptosis of RASFs.The expression of matrix metalloproteinase 3/13 ( MMP3/13) and interleukin 1β( IL-1β) was measured by RT-PCR and Western blotting.RESULTS: The proliferation and invasion of RASFs were signifi-cantly inhibited by miR-16 mimic.The result of flow cytometry demonstrated that miR-16 had no effect on apoptosis of RASFs.Furthermore, miR-16 down-regulated the expression of MMP3/13 and IL-1β.CONCLUSION:miR-16 plays an important role in the development of RA and may inhibit the proliferation and invasion of RASFs through down-regulating the expression of MMP3/13 and IL-1β.
