CAJ | 학술논문

目的：研究葡萄多酚对雨蛙肽诱导的小鼠急性胰腺炎（ AP）的保护作用。方法：2月龄ICR雌鼠共21只，随机分为正常对照组（ NC组）、AP模型组（ AP组）和药物处理AP组，每组7只。造模前，药物处理AP组小鼠连续7 d胃内灌注葡萄多酚（1．5 g/kg）水溶液，NC组和AP组小鼠则以同法给予生理盐水作为对照。第7天开始造模。 AP组以及药物处理AP组小鼠予以腹腔注射雨蛙肽（50μg/kg）造模，每小时1次，共注射7次；NC组小鼠同法注射等量生理盐水。于24 h处死小鼠取其胰腺及肺组织。测量各组胰腺组织的相对重量、病理形态学改变、巨噬细胞的浸润情况及炎症因子、氧化应激分子的表达水平；检测肺脏组织的髓过氧化物酶（MPO）活性以考察远隔器官的炎症。结果：与AP组小鼠相比，药物处理AP组小鼠胰腺组织的水肿、炎症以及空泡的评分明显降低（P＜0.05），但坏死和病理总评分没有明显变化；巨噬细胞的浸润明显减少；肿瘤坏死因子α（TNF-α）和单核细胞趋化蛋白1（MCP-1）的表达水平也明显降低（P＜0.05）；超氧化物歧化酶1（SOD-1）、SOD-2和NADPH氧化酶2（NOX-2）的表达水平也明显降低（P＜0.05， P＜0.05， P＜0.01）。此外，药物处理AP组小鼠肺组织的MPO活性显著降低（P＜0.01），提示中性粒细胞的浸润减少。结论：葡萄多酚对雨蛙肽诱导的小鼠急性胰腺炎胰腺组织具有明显保护作用，机制与下调炎症因子和氧化应激分子的表达有关。
목적：연구포도다분대우와태유도적소서급성이선염（ AP）적보호작용。방법：2월령ICR자서공21지，수궤분위정상대조조（ NC조）、AP모형조（ AP조）화약물처리AP조，매조7지。조모전，약물처리AP조소서련속7 d위내관주포도다분（1．5 g/kg）수용액，NC조화AP조소서칙이동법급여생리염수작위대조。제7천개시조모。 AP조이급약물처리AP조소서여이복강주사우와태（50μg/kg）조모，매소시1차，공주사7차；NC조소서동법주사등량생리염수。우24 h처사소서취기이선급폐조직。측량각조이선조직적상대중량、병리형태학개변、거서세포적침윤정황급염증인자、양화응격분자적표체수평；검측폐장조직적수과양화물매（MPO）활성이고찰원격기관적염증。결과：여AP조소서상비，약물처리AP조소서이선조직적수종、염증이급공포적평분명현강저（P＜0.05），단배사화병리총평분몰유명현변화；거서세포적침윤명현감소；종류배사인자α（TNF-α）화단핵세포추화단백1（MCP-1）적표체수평야명현강저（P＜0.05）；초양화물기화매1（SOD-1）、SOD-2화NADPH양화매2（NOX-2）적표체수평야명현강저（P＜0.05， P＜0.05， P＜0.01）。차외，약물처리AP조소서폐조직적MPO활성현저강저（P＜0.01），제시중성립세포적침윤감소。결론：포도다분대우와태유도적소서급성이선염이선조직구유명현보호작용，궤제여하조염증인자화양화응격분자적표체유관。
AIM:To investigate the effects of grape polyphenol ( GP) on caerulein-induced acute pancreatitis (AP) in mice.METHODS:Two-month-old female mice of ICR strains (n=21) were randomly divided into 3 groups:normal control ( NC) group, AP group, and GP-treated AP group.Before AP induction, the mice in GP-treated AP group were continuously administrated with 1.5 g/kg GP aqueous solution by gavage for 7 d, while those in NC group and AP group were treated with saline as a vehicle control.On the 7th day, the mice in AP group and GP-treated AP group were in-traperitoneally injected with caerulein (50μg/kg) in 1 h interval for 7 serial injections in total.The mice in NC group were treated with saline according to the same procedure in experimental group.All the mice were sacrificed 24 h after AP induc-tion, and the pancreatic tissues and lung tissues were harvested for further investigation of the pathological changes, macro-phages infiltration, myeloperoxidase ( MPO ) activity and expression of inflammatory and oxidative stress factors.RE-SULTS:Compared with AP group, the mice in GP-treated AP group showed milder morphological changes and lower path-ological scores, including the scores of edema, inflammation and vacuolization (P<0.05), but the necrosis scores and to-tal scores showed no statistical difference between these 2 groups.Besides, the mice in GP-treated AP group had fewer macrophage infiltration, lower lung MPO activity (P<0.01), and lower expression of inflammatory factors, tumor necrosis factor α(TNF-α)and monocyte chemotactic protein 1 (MCP-1) (P<0.05), and oxidative stress factors, superoxide dis-mutase (SOD)-1, SOD-2 and NADPH oxidase 2 (NOX-2) (P<0.01).CONCLUSION:Grape polyphenol has remark-able protective effect on pancreatic tissues of mice with caerulein-induced acute pancreatitis, and the mechanisms may be related to down-regulation of inflammatory and oxidative stress factors.