CAJ | 학술논문

目的：研究蜂毒肽（Melittin）对人前列腺癌PC-3细胞凋亡的影响及可能机制。方法实验设立空白对照组和蜂毒肽不同浓度组，用MTT方法检测细胞增殖，Hoechst 33258染色检测细胞凋亡，RT-PCR检测p27、p53 mRNA表达，Western blot检测蛋白表达。结果与对照组比较，蜂毒肽作用24、48、72 h后，4、8、16、32μg/mL组MTT检测OD值明显降低，细胞凋亡率明显增高（P＜0.05），作用48h后，p27、p53 mRNA表达明显升高（P＜0.05），p53和 Caspase3蛋白含量增高。结论蜂毒肽对PC-3细胞有凋亡诱导作用，其作用机制可能与增高p27、p53、Caspase3表达相关。
목적：연구봉독태（Melittin）대인전렬선암PC-3세포조망적영향급가능궤제。방법실험설립공백대조조화봉독태불동농도조，용MTT방법검측세포증식，Hoechst 33258염색검측세포조망，RT-PCR검측p27、p53 mRNA표체，Western blot검측단백표체。결과여대조조비교，봉독태작용24、48、72 h후，4、8、16、32μg/mL조MTT검측OD치명현강저，세포조망솔명현증고（P＜0.05），작용48h후，p27、p53 mRNA표체명현승고（P＜0.05），p53화 Caspase3단백함량증고。결론봉독태대PC-3세포유조망유도작용，기작용궤제가능여증고p27、p53、Caspase3표체상관。
Objective To study the effects of Melittin (Mel) on apoptosis of prostate cancer cell PC-3 and explore its potential mechanism. Methods Blank control Group and Mel groups of different concentrations were set up in this study. Cell proliferation was analyzed by MTT assay. Cell apoptosis was measured by Hoechst 33258 dying method. The expressions of p27and p53were detected by RT-qPCR, and their protein expressions were detected by Western blot. Results Compared with those of the controls, cell proliferation (OD value) decreased and apoptosis increased significantly in Mel groups of 4, 8, 16, 32μg/mL after 24, 48 and 72 h treatment. After 48h treatment, the expressions of p27 andp53 mRNA were increased significantly, and protein expression of p53 and caspase3 were also increased. Conclusion Mel may induce PC-3 cell apoptosisby upregulation of p27, p53 and caspase3 expression.