CAJ | 학술논문

目的：探讨在过氧化氢（ H2 O2）刺激下，左卡尼汀对心肌细胞内胞浆活化 T 细胞核因子3（NFATc3）的表达及分布的影响。方法：以200μmol/L H2O2刺激心肌细胞12 h建立氧化应激模型。在药物处理组，于氧化刺激前30 min加入左卡尼汀及钙调神经磷酸酶（ CaN）特异性抑制剂环孢素A（ CsA）。待刺激完成后，利用蛋白质免疫印迹法检测细胞内总NFATc3，以及胞浆内和胞核内NFATc3的水平，并以免疫荧光法检测NFATc3在细胞中的分布状况。结果：H2 O2刺激不影响心肌细胞NFATc3的表达，但可诱导其由胞浆至胞核的转位。左卡尼汀预处理可以显著抑制NFATc3的核转位。结论：左卡尼汀可通过抑制NFATc3的核转位发挥其抗氧化应激损伤的作用。
목적：탐토재과양화경（ H2 O2）자격하，좌잡니정대심기세포내포장활화 T 세포핵인자3（NFATc3）적표체급분포적영향。방법：이200μmol/L H2O2자격심기세포12 h건립양화응격모형。재약물처리조，우양화자격전30 min가입좌잡니정급개조신경린산매（ CaN）특이성억제제배포소A（ CsA）。대자격완성후，이용단백질면역인적법검측세포내총NFATc3，이급포장내화포핵내NFATc3적수평，병이면역형광법검측NFATc3재세포중적분포상황。결과：H2 O2자격불영향심기세포NFATc3적표체，단가유도기유포장지포핵적전위。좌잡니정예처리가이현저억제NFATc3적핵전위。결론：좌잡니정가통과억제NFATc3적핵전위발휘기항양화응격손상적작용。
AIM: To explore the effect of L-carnitine on nuclear factor of activated T-cells, cytoplasmic 3 (NFATc3) in cardiomyocytes under H2O2 stimulation.METHODS: Primary cultured neonatal rat myocardial cells were stimulated by H2 O2 at concentration of 200μmol/L for 12 h to induce oxidative stress injury.In treatment group, L-carni-tine and cyclosporin A ( CsA) , a specific inhibitor of calcineurin ( CaN) , were administered 30 min prior to H2 O2 stimula-tion.After treatment, total, cytoplasmic and nuclear NFATc3 protein levels were determined by Western blotting.The method of immunofluoresence was used to evaluate the distribution of NFATc3.RESULTS: H2 O2 treatment produced no effect on the expression of total NFATc3, but caused its translocation from the cytosolic to nuclear compartment, which was greatly blunted by L-carnitine pretreatment.CONCLUSION:L-carnitine antagonized oxidative stress injury via alleviating NFATc3 nuclear translocation.