CAJ | 학술논문

目的：利用表达μ阿片受体的中国仓鼠卵巢细胞，研究吗啡急慢性处理下对细胞外信号调节激酶（ ERK）磷酸化的影响。方法免疫印迹检测磷酸化ERK水平，获取1 h急性处理的ERK磷酸化变化时程和36 h慢性处理以及纳洛酮催促下的ERK磷酸化变化。结果1μmol·L-1吗啡可以快速诱导ERK磷酸化水平短暂升高，5 min时达峰（ P〈0.01），吗啡诱导的ERK磷酸化水平升高具有显著的浓度依赖性。10μmol·L-1吗啡慢性处理36 h后ERK磷酸化水平与对照组比较，差异无统计学意义，但纳洛酮急性催促5或10 min均导致ERK磷酸化显著下降（与对照比较，P〈0.01）。结论吗啡急慢性刺激下以及纳洛酮催促下ERK磷酸化表现出不同的变化形式，提示μ阿片受体介导下ERK相关的信号通路发生了代偿。
목적：이용표체μ아편수체적중국창서란소세포，연구마배급만성처리하대세포외신호조절격매（ ERK）린산화적영향。방법면역인적검측린산화ERK수평，획취1 h급성처리적ERK린산화변화시정화36 h만성처리이급납락동최촉하적ERK린산화변화。결과1μmol·L-1마배가이쾌속유도ERK린산화수평단잠승고，5 min시체봉（ P〈0.01），마배유도적ERK린산화수평승고구유현저적농도의뢰성。10μmol·L-1마배만성처리36 h후ERK린산화수평여대조조비교，차이무통계학의의，단납락동급성최촉5혹10 min균도치ERK린산화현저하강（여대조비교，P〈0.01）。결론마배급만성자격하이급납락동최촉하ERK린산화표현출불동적변화형식，제시μ아편수체개도하ERK상관적신호통로발생료대상。
Objective To study the phosphorylation mode of extracellular regulated kinase( ERK)induced by acute and chronic morphine treatment on Chinese hamster ovary( CHO)cells expressed withμopioid receptors. Methods The time course of ERK phosphorylation 1 h and 36 h after morphine exposure as well as naloxone-precipitated withdrawal was detected by immunobloting. Results A transient enhancement of ERK phosphorylation was induced by 1 μmol · L-1 morphine with the peak effect at 5 min(P〈0. 01),and the effect was dose-dependent. No difference in ERK phosphorylation was found after 36h of treatment with 10 μmol · L-1 morphine compared with the control. However,5 or 10 min-naloxone precipitation induced remarkable decrease in ERK phosphorylation compared with the control(P〈0. 01). Conclusion Different changes of ERK phosphorylation were found under acute and chronic morphine treatment and naloxone precipitation,indicating a compensation of ERK related pathway induced byμ opioid receptors.