CAJ | 학술논문

目的：探讨白藜芦醇（ Res）联合体外放射治疗（放疗）对肝癌Bel-7404细胞增殖、侵袭、凋亡的影响。方法25μmol·L-1 Res联合放疗处理肝癌Bel-7404细胞，并将细胞分为4组：对照组，2 Gy放疗组，4 Gy放疗组，6 Gy放疗组，噻唑蓝（ MTT）法检测肿瘤细胞的增殖及运动侵袭能力，荧光显微镜检测细胞凋亡，Western-blot法检测细胞基质金属蛋白酶2（MMP-2）、血管内皮生长因子（VEGF）蛋白表达情况。结果与对照组比较，2 Gy放疗组、4 Gy放疗组、6 Gy放疗组肝癌Bel-7404细胞增殖能力及侵袭能力均下降，凋亡细胞增多（ P〈0.05）。结论 Res能增强肝癌Bel-7404细胞对放疗的敏感性，放疗联合Res可使肝癌细胞增殖受抑，侵袭减弱，凋亡增加，其原因与降低MMP-2、VEGF蛋白表达有关。
목적：탐토백려호순（ Res）연합체외방사치료（방료）대간암Bel-7404세포증식、침습、조망적영향。방법25μmol·L-1 Res연합방료처리간암Bel-7404세포，병장세포분위4조：대조조，2 Gy방료조，4 Gy방료조，6 Gy방료조，새서람（ MTT）법검측종류세포적증식급운동침습능력，형광현미경검측세포조망，Western-blot법검측세포기질금속단백매2（MMP-2）、혈관내피생장인자（VEGF）단백표체정황。결과여대조조비교，2 Gy방료조、4 Gy방료조、6 Gy방료조간암Bel-7404세포증식능력급침습능력균하강，조망세포증다（ P〈0.05）。결론 Res능증강간암Bel-7404세포대방료적민감성，방료연합Res가사간암세포증식수억，침습감약，조망증가，기원인여강저MMP-2、VEGF단백표체유관。
Objective To investigate the effects of radiotherapy combined with resveratrol( Res)on proliferation, invasion and apoptosis of hepatoma cell line Bel-7404. Methods Bel-7404 cell line was treated with Res(25 μmol·L-1 ) combined with radiotherapy,then divided into four groups( group 1:the control;group 2:radiation at dose of 2 Gy;group 3:radiation at dose of 4 Gy;group 4:radiation at dose of 6 Gy). Cell proliferation and invasion were detected by MTT assay. Cell apoptosis were observed by fluorescence microscopy. Expressions of MMP-2 and VEGF proteins were determined by Western blotting. Results Compared with the control group,cell proliferation and invasion were significantly inhibited,while cell apoptosis was increased in all radiation groups(P〈0. 05). Conclusion The sensitivity of hepatoma Bel-7404 cells to radiation can be enhanced by resveratrol. Radiation therapy combined with resveratrol can inhibit proliferation and invasion of hepatoma cells and increase the cell apoptosis,which may be related with the down-regulation of MMP-2 and VEGF proteins.