CAJ | 학술논문

目的：研究松生等总黄酮的最佳优化提取工艺，并对不同极性溶剂提取部分的镇痛抗炎作用进行研究。方法通过单因素实验和正交实验，考察提取时间、料液比、乙醇浓度和提取次数对松生等总黄酮提取率的影响；采用小鼠冰醋酸扭体法及二甲苯致小鼠耳肿胀模型筛选松生等不同溶剂提取部分镇痛抗炎作用。结果回流提取法提取松生等总黄酮的最佳条件为：提取时间3 h、料液比1：30、乙醇浓度60%、提取2次，黄酮提取率最高5.63%；松生等乙醇提取物、正丁醇提取物和乙酸乙酯提取物均能够抑制冰醋酸引起的小鼠扭体反应和二甲苯引起的小鼠耳肿胀。结论该工艺提取松生等总黄酮提取率高，方法简便，重复性好。松生等乙醇提取物、正丁醇提取物和乙酸乙酯提取物均具有明显镇痛抗炎作用。
목적：연구송생등총황동적최가우화제취공예，병대불동겁성용제제취부분적진통항염작용진행연구。방법통과단인소실험화정교실험，고찰제취시간、료액비、을순농도화제취차수대송생등총황동제취솔적영향；채용소서빙작산뉴체법급이갑분치소서이종창모형사선송생등불동용제제취부분진통항염작용。결과회류제취법제취송생등총황동적최가조건위：제취시간3 h、료액비1：30、을순농도60%、제취2차，황동제취솔최고5.63%；송생등을순제취물、정정순제취물화을산을지제취물균능구억제빙작산인기적소서뉴체반응화이갑분인기적소서이종창。결론해공예제취송생등총황동제취솔고，방법간편，중복성호。송생등을순제취물、정정순제취물화을산을지제취물균구유명현진통항염작용。
Objective The study aimed to investigate the best extraction process and the analgesia and anti-inflammatory properties of total flavonoids from Euphorbia prolifera. Methods Through single factor experiment and orthogonal test,factors that affect the extraction yield of total flavonoids were studied,including the extraction time,solid to liquid ratio, ethanol concentration and extraction times. The mice models of ear edema induced by xylene and twisting induced by acetic acid were used to evaluate the analgesia and anti-inflammatory effects of different extractions from Euphorbia prolifera. Results Total flavonoids were extracted by the refluxing method,and the optimum conditions were extracting for 3 hours,solid to liquid ratio of 1:30,ethanol content of 60% in the solvent,and processed for 2 times. The highest extraction yield of total flavonoids from Euphorbia prolifera was 5. 63%. The ethanol,ethyl acetate and n-butanol extracts decreased twisting and ear swelling in mice. Conclusion The extraction for total flavonoids from Euphorbia prolifera is simple,efficient and reproducible. The ethanol,ethyl acetate and n-butanol extracts of total flavonoids have obvious analgesia and anti-inflammatory effects.