CAJ | 학술논문

目的：分析ROS1基因重排与胃腺癌临床病理学指标的关系。方法应用荧光原位杂交技术（FISH），检测60例胃腺癌肿瘤组织中ROS1基因的重排；采用siRNA和Real-time PCR检测ROS1重排与胃癌细胞免疫反应的关键分子的关系。结果胃腺癌组织中ROS1基因重排发生率为1．7％（1/60）。 ROS1基因高表达与胃肿瘤淋巴结转移呈正相关（P＜0．05），但与患者性别、年龄、发生部位、肿瘤直径、Laurén分类、病理分级、HP感染、浸润深度和TNM 分期等无关。 ROS1阳性表达肿瘤组织中IL-6、TNF-α阳性表达率明显高于ROS1阴性组织。结论 ROS1基因重排可能与炎症因子基因协同发挥促癌作用。
목적：분석ROS1기인중배여위선암림상병이학지표적관계。방법응용형광원위잡교기술（FISH），검측60례위선암종류조직중ROS1기인적중배；채용siRNA화Real-time PCR검측ROS1중배여위암세포면역반응적관건분자적관계。결과위선암조직중ROS1기인중배발생솔위1．7％（1/60）。 ROS1기인고표체여위종류림파결전이정정상관（P＜0．05），단여환자성별、년령、발생부위、종류직경、Laurén분류、병리분급、HP감염、침윤심도화TNM 분기등무관。 ROS1양성표체종류조직중IL-6、TNF-α양성표체솔명현고우ROS1음성조직。결론 ROS1기인중배가능여염증인자기인협동발휘촉암작용。
Objective To investigate the relationship between ROS 1 rearrangements and clinicopathological indicators in gastric adenocarcinomas. Methods ROS1 rearrangements in 60 cases of gastric adenocarcinoma tissues were detected by fluores-cence in situ hybridization ( FISH), and the relationship between ROS 1 rearrangements and key moleculars of immune response in gastric cancer were detected by siRNA and RT-PCR.Results The incidence rate of ROS1 gene rearrangement in gastric cancer was 1.7%(1/60).And the high expression of ROS1 was positively related with lymph node metastasis in gastric tumors, but had no correlation with gender, age,location,diameter,Laur én classification,pathological grade,HP infection,depth of invasion and TNM stageing(P>0.05).But which were correlated with lymph node metastasis (P<0.05).The positive expression of IL-6 and TNF-αin the ROS1 positive group was significantly higher than that of ROS 1 negative group. Conclusion ROS1 gene rearrange-ment may collaborate with inflammatory cytokines gene on tumor promotion.