CAJ | 학술논문

为测定栽培蓝莓－园蓝果实中的抗氧化活性及其总花色苷含量，以贵州产园蓝鲜果为原料，经甲醇处理、大孔吸附树脂分离得出抗氧化活性最好的部位。然后对其进行了制备分离得到一单体化合物，经1H－NMR、13C－NMR、ESI－MS进行了结构鉴定，利用Rp－HPLC进行了该化合物的含量测定。以该化合物为对照品，对园蓝果实中总花色苷含量进行了定量分析。研究表明：贵州产园蓝提取物富含花青素部分1mg/mL浓度的抗氧化活性与等浓度的维生素C的活性相当，鉴定的单体化合物为锦葵色素－3－O －葡萄糖，其纯度达96％，园蓝鲜果中总花青素平均含量为627．7mg/100g。指出了园蓝鲜果花色苷含量高，主成分为锦葵色素－3－O －葡萄糖，花青素具有较好的抗氧化活性。建议每人每天食用2g园蓝鲜果作为人体补充抗氧化剂的来源。
위측정재배람매－완람과실중적항양화활성급기총화색감함량，이귀주산완람선과위원료，경갑순처리、대공흡부수지분리득출항양화활성최호적부위。연후대기진행료제비분리득도일단체화합물，경1H－NMR、13C－NMR、ESI－MS진행료결구감정，이용Rp－HPLC진행료해화합물적함량측정。이해화합물위대조품，대완람과실중총화색감함량진행료정량분석。연구표명：귀주산완람제취물부함화청소부분1mg/mL농도적항양화활성여등농도적유생소C적활성상당，감정적단체화합물위금규색소－3－O －포도당，기순도체96％，완람선과중총화청소평균함량위627．7mg/100g。지출료완람선과화색감함량고，주성분위금규색소－3－O －포도당，화청소구유교호적항양화활성。건의매인매천식용2g완람선과작위인체보충항양화제적래원。
The Objective of the article is to determine the antioxidant activity and quantification of anthocya‐nin in the cultivated blueberry - -gardenblue .The Method of the experiment is that taking the fresh fruit of gardenblue cultivated in Guizhou province as raw material and extracting the part of the best antioxidant ac‐tivity through methanol treatment and macroreticular resin .Then ,the preparative separations of part of the best antioxidant activity gets monomeric compound which is tested 1 H -NMR ,13 C -NMR and ESI -MS , and quantitative analysis of the monomeric compound is done based on Rp -HPLC .Considering the com‐pound as a comparison product ,the experiment makes a quantitative analysis of total anthocyanidins .The re‐sults show that in the extracted part which contains anthocyanidins ,1mg/mL concentration's antioxidant ac‐tivity is equivalent to that of the same concentration of Vitamin C .T he appraised monomeric compound is malvidin -3-O -glucose whose purity reaches 96% .The average content of total anthocyanin of gardenblue's fresh fruits is 627 .7mg/100g .The conclusion is that the gardenblue contains the richest anthocyanin whose main component is malvidin 3-O -glucose ,and ,anthocyanin shows a better antioxidant activity .Therefore , the article advises that people should consume 2 g fresh fruits of gardenblue everyday as the source of antiox‐idant .