实用药物与临床
實用藥物與臨床
실용약물여림상
PRACTICAL PHARMACY AND CLINICAL REMEDIES
2014年
11期
1453-1455
,共3页
加红生化汤%阿魏酸%含量测定%高效液相色谱法
加紅生化湯%阿魏痠%含量測定%高效液相色譜法
가홍생화탕%아위산%함량측정%고효액상색보법
Jiahong Shenghuatang%Ferulic acid%Content determination%HPLC
目的：探讨加红生化汤中阿魏酸的含量测定方法。方法采用高效液相色谱法,用Agilent TC-C18(4．6 mm ×250 mm,5μm)色谱柱,用甲醇-0．3%冰醋酸溶液(30∶70)作为流动相,流速为1．0 mL/min,检测波长为323 nm,进样量10μL。结果阿魏酸在浓度9．88~98．8μg/mL范围内与峰面积形成良好的线性关系,线性方程为Y=57765 X-47．898(R2=0．9998)；平均回收率为99．07%,RSD为1．4%(n=6)。结论该方法简便、快速、准确、重复性好,可作为加红生化汤中阿魏酸的含量测定方法。
目的：探討加紅生化湯中阿魏痠的含量測定方法。方法採用高效液相色譜法,用Agilent TC-C18(4．6 mm ×250 mm,5μm)色譜柱,用甲醇-0．3%冰醋痠溶液(30∶70)作為流動相,流速為1．0 mL/min,檢測波長為323 nm,進樣量10μL。結果阿魏痠在濃度9．88~98．8μg/mL範圍內與峰麵積形成良好的線性關繫,線性方程為Y=57765 X-47．898(R2=0．9998)；平均迴收率為99．07%,RSD為1．4%(n=6)。結論該方法簡便、快速、準確、重複性好,可作為加紅生化湯中阿魏痠的含量測定方法。
목적：탐토가홍생화탕중아위산적함량측정방법。방법채용고효액상색보법,용Agilent TC-C18(4．6 mm ×250 mm,5μm)색보주,용갑순-0．3%빙작산용액(30∶70)작위류동상,류속위1．0 mL/min,검측파장위323 nm,진양량10μL。결과아위산재농도9．88~98．8μg/mL범위내여봉면적형성량호적선성관계,선성방정위Y=57765 X-47．898(R2=0．9998)；평균회수솔위99．07%,RSD위1．4%(n=6)。결론해방법간편、쾌속、준학、중복성호,가작위가홍생화탕중아위산적함량측정방법。
Objective To discuss a method for the content determination of ferulic acid in Jiahong Shenghua-tang. Methods The content of ferulic acid was determined by HPLC. The separation was performed on Agilent TC-C18 (4. 6 mm × 250 mm,5 μm). The mobile phase consisted of Acetonitrile-0. 3%phosphoric acid solution(30∶70),the flow rate was 1. 0 mL/min and the detection wavelength was at 323 nm. Results The linear range was 9. 88 ~98. 8μg/mL,and the regression equation was Y=57 765 X-47. 898(R2 =0. 999 8). The average recovery was 99. 07%and RSD was 1. 4%(n=6). Conclusion The method is simple,accurate and reproducible,which can be used for the con-tent determination of ferulic acid in Jiahong Shenghuatang.
