CAJ | 학술논문

从内蒙古某处土壤中分离出一株分解尿素的产氨细菌(命名为 JAT-1),革兰氏阴性,菌落表面平整湿润,乳白色,短杆状.菌体尺寸为φ0.2~0.4μm ×1.5~2.0μm.结合16S rDNA 测定和菌株系统发育树分析,其与 Providencia Sp. Sam 130-9A 同源性高达99%.对其生长特性研究结果表明：JAT-1以10 g·L -1柠檬酸钠作为碳源,20 g·L -1尿素作为氮源,最佳生长pH 值范围为8.0~9.5,最佳初始接种浓度20%.采用该细菌进行碱性铜矿摇瓶浸出实验,144 h 后铜浸出率可达42.38%,表明该菌株具有应用于碱性铜矿浸出的潜力.
종내몽고모처토양중분리출일주분해뇨소적산안세균(명명위 JAT-1),혁란씨음성,균락표면평정습윤,유백색,단간상.균체척촌위φ0.2~0.4μm ×1.5~2.0μm.결합16S rDNA 측정화균주계통발육수분석,기여 Providencia Sp. Sam 130-9A 동원성고체99%.대기생장특성연구결과표명：JAT-1이10 g·L -1저몽산납작위탄원,20 g·L -1뇨소작위담원,최가생장pH 치범위위8.0~9.5,최가초시접충농도20%.채용해세균진행감성동광요병침출실험,144 h 후동침출솔가체42.38%,표명해균주구유응용우감성동광침출적잠력.
An ammonia-producing strain isolated from Inner Mongolia in north China, which is capable of decomposing urea, was designated as strain JAT- 1. Identification results show that this milky strain has a smooth and humid surface, and it is Gram-negative and rod-shaped in φ0. 2 - 0. 4 μm × 1. 5 - 2. 0 μm. Based on the 16S rDNA sequencing and the phylogenetic tree, this strain was iden-tified as Providencia Sp. By means of the analysis of growth characteristics, the optimal growth condition was achieved when the con-centration of sodium citrate as a carbon source was 10 g·L - 1 , the concentration of urea as a nitrogen source was 20 g·L - 1 , the pH value was between 8. 0 and 9. 5, and the inoculum concentration was 20% . Alkaline copper ore bioleaching experiment in a shake flask with the ammonia-producing strain yielded a copper leaching efficiency of 42. 38% in 144 h. This result indicates that this strain has the po-tential for leaching the alkaline copper ore.