CAJ | 학술논문

目的：研究不同醇沉竹节参多糖的免疫佐剂活性，探讨其中佐剂活性最强的部分。方法通过对竹节参总多糖进行不同浓度醇沉，得到30%醇沉物、60%醇沉多糖和90%醇沉多糖，将各段多糖分别与卵白蛋白混合注入小鼠体内进行免疫，每周免疫1次，共3次。最后1次免疫后5d处死小鼠取血，测定抗体效价。对3种醇沉物质进行红外光谱扫描，初步确定多糖的类型。结果各段多糖的抗体效价与对照组比较均显著增加，尤以60%醇沉多糖最为明显。红外图谱分析表明，竹节参多糖的骨架中含有吡喃糖环结构。结论竹节参多糖的免疫佐剂效果显著，其中60%醇沉多糖的佐剂效果最强。
목적：연구불동순침죽절삼다당적면역좌제활성，탐토기중좌제활성최강적부분。방법통과대죽절삼총다당진행불동농도순침，득도30%순침물、60%순침다당화90%순침다당，장각단다당분별여란백단백혼합주입소서체내진행면역，매주면역1차，공3차。최후1차면역후5d처사소서취혈，측정항체효개。대3충순침물질진행홍외광보소묘，초보학정다당적류형。결과각단다당적항체효개여대조조비교균현저증가，우이60%순침다당최위명현。홍외도보분석표명，죽절삼다당적골가중함유필남당배결구。결론죽절삼다당적면역좌제효과현저，기중60%순침다당적좌제효과최강。
Objective To study the adjuvant immunoactivity of polysaccharides from Panax japonicus by alcohol of different concentrations;To discuss its part with the strongest adjuvant immunoactivity. Methods Polysaccharides from Panax japonicus was sunk with alcohol of different concentrations, and 30%alcohol compound, 60%and 90%alcohol polysaccharide were obtained. Different segments of polysaccharide and OVA protein were injected to mice once a week for three times for immunity. Five days after the last immunity, the mice were executed to collect blood, and the antibody titer was determined. The three parts of alcohol compound were scanned by infrared spectrum to determine the type of polysaccharide preliminarily. Results Compared with the control group, the antibody titer of different segments of polysaccharide obviously increased, especially the polysaccharide sunk by 60%alcohol. Infrared spectrum analysis showed that polysaccharides from Panax japonicus contained pyranose ring structure. Conclusion Polysaccharides from Panax japonicus has significant adjuvant immunoactivity, and polysaccharide sunk by 60%alcohol has the strongest adjuvant effects.