CAJ | 학술논문

目的：对比硫磺熏蒸前后生姜高效液相色谱(HPLC)指纹图谱结构差异,探讨硫磺熏蒸对生姜质量的影响。方法采用 HPLC 二极管阵列检测器,ZORBAX SB-C18柱(4.6 mm×150 mm,5μm),乙腈-水为流动相,梯度洗脱,体积流量1 mL/min,柱温25℃,检测波长280 nm,建立硫磺熏蒸前后生姜的化学成分指纹图谱,对硫磺熏蒸前后的HPLC图谱进行成分分析,并采用外标法对活性成分6-姜酚进行定量检测。结果经过比较3批生姜硫熏品与3批生姜非硫熏品的指纹图谱,建立了17个共有色谱峰,3号色谱峰的峰面积下降了50.68%,10号峰下降了64.41%,11号峰下降了67.68%,17号下降了21.23%。主要活性成分6-姜酚含量无显著变化。结论生姜经过硫磺熏蒸后,其化学成分组成结构及主要活性成分含量均发生改变,其安全性、有效性有待进一步商榷。
목적：대비류광훈증전후생강고효액상색보(HPLC)지문도보결구차이,탐토류광훈증대생강질량적영향。방법채용 HPLC 이겁관진렬검측기,ZORBAX SB-C18주(4.6 mm×150 mm,5μm),을정-수위류동상,제도세탈,체적류량1 mL/min,주온25℃,검측파장280 nm,건립류광훈증전후생강적화학성분지문도보,대류광훈증전후적HPLC도보진행성분분석,병채용외표법대활성성분6-강분진행정량검측。결과경과비교3비생강류훈품여3비생강비류훈품적지문도보,건립료17개공유색보봉,3호색보봉적봉면적하강료50.68%,10호봉하강료64.41%,11호봉하강료67.68%,17호하강료21.23%。주요활성성분6-강분함량무현저변화。결론생강경과류광훈증후,기화학성분조성결구급주요활성성분함량균발생개변,기안전성、유효성유대진일보상각。
Objective To explain the differences between zingiber officinale and its sulfur fumigation products on chromatography fingerprints by HPLC-DAD;To discuss the influence of sulfur-fumigation on the quality of zingiber officinale. Methods HPLC, diode array detector, and ZORBAX SB-C18 column were used with acetonitrile-water as the mobile phase, gradient elute, volume flow rate of 1 mL/min, column temperature of 25 ℃, and detection wavelength of 280 nm. HPLC-DAD technology was applied to establish the fingerprints of zingiber officinale before and after sulfur-fumigating process, in order to analyze the HPLC fingerprints of zingiber officinale before and after sulfur-fumigating process. External standard method was used to do the quantitative determination of 6-gingerol. Results The 17 common peaks were identified through the comparison of 3 batches of fingerprints of zingiber officinale and their sulfur-fumigated samples. The peak areas of NO.3, NO.10, NO.11, and NO.17 were reduced by 50.68%, 64.41%, 67.68%, and 21.23%respectively. The content of 6-gingerol had no significant change. Conclusion The chemical composition of zingiber officinale changed at different degrees after sulfur-fumigated process. The safety and effectiveness of sulfur fumigation products of zingiber officinale require more researches.