CAJ | 학술논문

目的：比较不同产地栽培与野生喜马拉雅紫茉莉中所含葫芦巴碱的差异。方法采用高效液相色谱法测定喜马拉雅紫茉莉中葫芦巴碱的含量。采用ZORBAX XDB-CN 色谱柱(4.6 mm×250 mm,5μm)；流动相：乙腈-0.03%冰醋酸(85∶15)；流速：0.8 mL/min；检测波长：265 nm,参比波长：360 nm；柱温：30℃。结果以峰面积对对照品溶液浓度进行回归计算,得回归方程 A＝23.409C－26.398,r＝0.9998,葫芦巴碱在2.004～200.400μg/mL 范围内与峰面积线性关系良好,葫芦巴碱的平均回收率为99.57%,RSD＝1.11%。结论不同产地栽培与野生喜马拉雅紫茉莉中葫芦巴碱含量无明显差异,本研究为栽培喜马拉雅紫茉莉药材的使用奠定了基础。
목적：비교불동산지재배여야생희마랍아자말리중소함호호파감적차이。방법채용고효액상색보법측정희마랍아자말리중호호파감적함량。채용ZORBAX XDB-CN 색보주(4.6 mm×250 mm,5μm)；류동상：을정-0.03%빙작산(85∶15)；류속：0.8 mL/min；검측파장：265 nm,삼비파장：360 nm；주온：30℃。결과이봉면적대대조품용액농도진행회귀계산,득회귀방정 A＝23.409C－26.398,r＝0.9998,호호파감재2.004～200.400μg/mL 범위내여봉면적선성관계량호,호호파감적평균회수솔위99.57%,RSD＝1.11%。결론불동산지재배여야생희마랍아자말리중호호파감함량무명현차이,본연구위재배희마랍아자말리약재적사용전정료기출。
Objective To compare differences and similarities of the content of trigonelline in Himalaica mirabilis of either wild or cultivated materials from different places. Methods An HPLC method was established to determine the content of trigonelline in Himalaica mirabilis. ZORBAX XDB-CN column (4.6 mm×250 mm, 5 μm) was used, with mobile phase of acetonitrile-0.03%acetic acid (85∶15), flow rate of 0.8 mL/min, detection wavelength of 265 nm, determination wavelength of 360 nm, and column temperature of 30 ℃. Results Regression calculation was made on peak area with the reference solution concentration, and then got the regression equation A=23.409C-26.398, r=0.999 8. Trigonelline showed good linear relation with peak area among the range of 2.004-200.400 μg/mL. The average recovery of trigonelline was 99.57%, RSD=1.11%. Conclusion There was no significant difference in the content of trigonelline of either wild or cultivated materials from different places. This study laid the foundation of application of the cultivated Himalaica mirabilis.