CAJ | 학술논문

目的：观察灯盏花胶囊对慢性肾功能衰竭(CRF)大鼠肾组织微炎症反应的影响,探讨其治疗CRF的可能机制。方法将60只SD大鼠随机分为正常组、模型组、贝那普利组和灯盏花组,每组15只,应用Platt 5/6肾切除方法制备CRF模型。贝那普利组予盐酸贝那普利(0.29 mg/100 g)灌胃,灯盏花组予灯盏花胶囊(0.3 g/100 g)灌胃,正常组及模型组予等量生理盐水灌胃,疗程12周。RT-PCR检测肾组织转化生长因子β1(TGF-β1)、纤溶酶原激活物抑制因子1(PAI-1)的表达。ELISA检测大鼠肾组织白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。结果与模型组比较,灯盏花组、贝那普利组大鼠肾组织TGF-β1、PAI-1水平下降,IL-6、TNF-α含量降低(P＜0.05),且灯盏花组较贝那普利组降低更加明显(P＜0.05)。结论灯盏花胶囊能减轻CRF大鼠肾组织的炎症反应,抑制肾组织纤维化。
목적：관찰등잔화효낭대만성신공능쇠갈(CRF)대서신조직미염증반응적영향,탐토기치료CRF적가능궤제。방법장60지SD대서수궤분위정상조、모형조、패나보리조화등잔화조,매조15지,응용Platt 5/6신절제방법제비CRF모형。패나보리조여염산패나보리(0.29 mg/100 g)관위,등잔화조여등잔화효낭(0.3 g/100 g)관위,정상조급모형조여등량생리염수관위,료정12주。RT-PCR검측신조직전화생장인자β1(TGF-β1)、섬용매원격활물억제인자1(PAI-1)적표체。ELISA검측대서신조직백세포개소-6(IL-6)、종류배사인자-α(TNF-α)수평。결과여모형조비교,등잔화조、패나보리조대서신조직TGF-β1、PAI-1수평하강,IL-6、TNF-α함량강저(P＜0.05),차등잔화조교패나보리조강저경가명현(P＜0.05)。결론등잔화효낭능감경CRF대서신조직적염증반응,억제신조직섬유화。
Objective To observe the effects of Dengzhanhua Capsule on kidney tissue inflammatory cytokines in chronic renal failure rats;To explore its possible mechanism for the efficacy in chronic renal failure. Methods Sixty SD rats were randomly divided into normal control group, model group, benazepril group and Dengzhanhua group, 15 rats in each group. Chronic renal failure rat model was established by Platt 5/6 nephrectomized. Benazepril (0.29 mg/100 g) was given to rats in the benazepril group by gastrogavage. Dengzhanhua Capsule (0.3 g/100 g) was given to rats in the Dengzhanhua group by gastrogavage. Normal saline was given to rats in the normal group and the model group by gastrogavage. The whole treatment period was twelve weeks. Expressions of TGF-β1 and PAI-1 were determined by semi-quantitative RT-PCR after treatment. Concentrations of kidney tissue inflammatory cytokines IL-6 and TNF-α were determined by ELISA. Results Expressions of TGF-β, PAI-1 and IL-6, TNF-αin benazepril group and Dengzhanhua group were significantly lower than those in model group (P<0.05). Compared with benazepril group, it was significantly lower in Dengzhanhua group (P<0.05). Conclusion Dengzhanhua Capsule can reduce kidney tissue inflammatory in chronic renal failure rats, and inhibit renal fibrosis.