CAJ | 학술논문

目的：观察叶下珠提取物在小鼠急性乙型肝炎病毒(HBV)感染模型中对HBV复制及其抗原表达的影响。方法 C57b/6小鼠尾静脉注射1.3倍HBV真核表达质粒(pHBV1.3),20μg/只,建立小鼠急性HBV感染模型。实验小鼠随机分为正常组、病毒组及中药高、中、低剂量组,感染后第1日起,给药组分别给予相应剂量药物灌胃,连续3 d。感染后第4日,化学发光法检测血清乙型肝炎表面抗原(HBsAg)、乙型肝炎e抗原(HBeAg)水平,PCR检测血清HBV-DNA含量；免疫组化法检测肝组织乙型肝炎核心抗原(HBcAg)、HBsAg表达。结果中药各剂量组均能明显降低感染小鼠血清中HBsAg、HBeAg含量,与病毒组比较差异有统计学意义(P＜0.01)；中药高剂量组显著降低血清中HBV-DNA水平,与病毒组比较差异有统计学意义(P＜0.01),中药中、低剂量组血清HBV-DNA含量降低,但与病毒组比较差异无统计学意义(P＞0.05)；中药高剂量组明显抑制了肝组织了 HBcAg 及 HBsAg的表达(P＜0.05)。结论叶下珠提取物明显抑制小鼠急性HBV感染模型小鼠HBV的复制与表达,具有直接抗病毒作用。
목적：관찰협하주제취물재소서급성을형간염병독(HBV)감염모형중대HBV복제급기항원표체적영향。방법 C57b/6소서미정맥주사1.3배HBV진핵표체질립(pHBV1.3),20μg/지,건립소서급성HBV감염모형。실험소서수궤분위정상조、병독조급중약고、중、저제량조,감염후제1일기,급약조분별급여상응제량약물관위,련속3 d。감염후제4일,화학발광법검측혈청을형간염표면항원(HBsAg)、을형간염e항원(HBeAg)수평,PCR검측혈청HBV-DNA함량；면역조화법검측간조직을형간염핵심항원(HBcAg)、HBsAg표체。결과중약각제량조균능명현강저감염소서혈청중HBsAg、HBeAg함량,여병독조비교차이유통계학의의(P＜0.01)；중약고제량조현저강저혈청중HBV-DNA수평,여병독조비교차이유통계학의의(P＜0.01),중약중、저제량조혈청HBV-DNA함량강저,단여병독조비교차이무통계학의의(P＞0.05)；중약고제량조명현억제료간조직료 HBcAg 급 HBsAg적표체(P＜0.05)。결론협하주제취물명현억제소서급성HBV감염모형소서HBV적복제여표체,구유직접항병독작용。
Objective To observe the effects of the extract from Phyllanthus urinaria (PU) on HBV replication and antigen expression in a murine model of acute hepatitis B virus infection. Methods The eukaryotic expression plasmid HBV1.3, which contained 1.3-fold-overlength genome of HBV, was transfected into C57b/6 mice by hydrodynamic injection via tail vein, to establish a murine model of acute hepatitis B virus infection. The mice were randomly divided into normal group, virus group and PU high, medium and low dosage group. The normal group was injected PBS 1.6 mL per mice. From the 1st day after infection, the mice were treated by intragastric administration with PU at different concentrations for 3 days. On the 4th day after infection, chemiluminescence method was used to detect the concentrations of HBsAg and HBeAg in mice serum;PCR method was used to detect the level of HBV-DNA in serum;immunohistochemistry was used to detect the expressions of HBcAg and HBsAg in mice liver. Results The PU high, medium and low dosage groups all reduced the concentrations of HBsAg and HBeAg in mice sera obviously, compared with the infected mice (P<0.01), in a dosage-dependent manner. The high dosage group dramatically reduced HBV-DNA level, compared with the virus group (P<0.01). The HBV-DNA concentration in medium and low dosage groups decreased, however, there were no significant difference (P>0.05). The drug in high dosage group obviously inhibited the HBcAg and HBsAg expressions in mice liver (P<0.05). Conclusion The PU extract obviously inhibited HBV replication and expression in mice model of acute HBV infection and exerted distinctive anti-HBV effects.