CAJ | 학술논문

目的：观察清热排毒胶囊对尿酸钠晶体(MSU)诱导的急性痛风性关节炎模型大鼠核因子κB(NF-κB)和白细胞介素-1β(IL-1β)的影响,探讨其防治急性痛风性关节炎的作用机理。方法60只SD雄性大鼠随机分为空白组、模型组、双氯芬酸钠缓释片组(戴芬组)及清热排毒胶囊高、中、低剂量组,各治疗组予相应药物灌胃7 d,于第5日灌胃给药1 h后采用MSU溶液踝关节腔注射制备大鼠急性痛风性关节炎模型。各组大鼠于造模后48 h处死并取材。光镜下观察各组大鼠踝关节滑膜组织的病理形态学改变,ELISA测定各组大鼠踝关节浸出液中NF-κB和IL-1β的表达水平。结果与模型组比较,清热排毒胶囊各剂量组和戴芬组大鼠踝关节浸出液中 NF-κB 和 IL-1β的表达水平均显著降低(P＜0.05,P＜0.01)。病理观察结果显示,清热排毒胶囊可减轻模型大鼠踝关节滑膜组织充血、水肿及炎性细胞浸润,并改善滑膜增生等病理改变。结论清热排毒胶囊通过抑制大鼠踝关节组织中NF-κB和IL-1β的表达来发挥抗炎作用。
목적：관찰청열배독효낭대뇨산납정체(MSU)유도적급성통풍성관절염모형대서핵인자κB(NF-κB)화백세포개소-1β(IL-1β)적영향,탐토기방치급성통풍성관절염적작용궤리。방법60지SD웅성대서수궤분위공백조、모형조、쌍록분산납완석편조(대분조)급청열배독효낭고、중、저제량조,각치료조여상응약물관위7 d,우제5일관위급약1 h후채용MSU용액과관절강주사제비대서급성통풍성관절염모형。각조대서우조모후48 h처사병취재。광경하관찰각조대서과관절활막조직적병리형태학개변,ELISA측정각조대서과관절침출액중NF-κB화IL-1β적표체수평。결과여모형조비교,청열배독효낭각제량조화대분조대서과관절침출액중 NF-κB 화 IL-1β적표체수평균현저강저(P＜0.05,P＜0.01)。병리관찰결과현시,청열배독효낭가감경모형대서과관절활막조직충혈、수종급염성세포침윤,병개선활막증생등병리개변。결론청열배독효낭통과억제대서과관절조직중NF-κB화IL-1β적표체래발휘항염작용。
Objective To observe the effects of Qingre Paidu Capsule on NF-κB and IL-1βin acute gouty arthritis model rats induced by monosodium urate crystals (MSU);To explore its preventive and therapeutic mechanism for acute gouty arthritis. Methods Sixty SD rats were randomly divided into normal group, model group, diclofenac sodium group, and Qingre Paidu Capsule high, medium and low dose groups. Rats in different treatment groups were given corresponding medicines by gavage for 7 days. On the fifth day, after 1 hour of gastric perfusion, acute gouty arthritis rat model was established by MSU injection in ankle joint cavity. All rats were sacrificed and materials were taken 48 h after the model was established. The morphological changes in the synovial tissue of ankle joint were observed by light microscope. The expression levels of NF-κB and IL-1β in the articular leachate were monitored by ELISA. Results The expression levels of NF-κB and IL-1βin the articular leachate of Qingre Paidu Capsule each dose group and diclofenac sodium group were obviously lower than the model group (P<0.05, P<0.01). Histopathologic examination revealed that Qingre Paidu Capsule could abate tissue swelling of ankle joints, reduce inflammatory cell infiltration of synovial tissues and improve the pathologic changes of the hyperplasia of synovium and so on. Conclusion Qingre Paidu Capsule play the anti-inflammatory role by inhibiting the expressions of NF-κB and IL-1βin articular tissue of rats.